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K4002

Sigma-Aldrich

Krebs-Ringer Bicarbonate Buffer

With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

Synonyme(s) :

Bicarbonate Buffer Solution, Krebs Buffer, Krebs-Ringer Solution

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About This Item

Code UNSPSC :
12161702
Nomenclature NACRES :
NA.75

Niveau de qualité

500

Forme

powder

Technique(s)

cell culture | mammalian: suitable

pH

6.1-6.7

Solubilité

water: 9.5 g/L, clear

Température de stockage

2-8°C

Description générale

Krebs-Ringer Bicarbonate Buffer acts as a salt solution and plays an essential role in tissue culture. A salt solution provides the cells with water and inorganic ions. It also helps in maintaining the osmotic balance and ideal pH in the medium. This bicarbonate buffered isotonic saline contains magnesium, potassium, sodium, and phosphate ions.

Application

Krebs-Ringer Bicarbonate Buffer has been used:

  • as a wash buffer for the preparation of mice primary adipocytes and lipolysis assay
  • in the pre-perifusion for islet viability and functionality studies
  • in pituitary bioassay

Quantité

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitution

Supplement with 1.26 g/L sodium bicarbonate.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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The Hypothalamic-Pituitary-Adrenal Axis Response to Stress in Mice Lacking Functional Vasopressin V1b Receptors
Stephen J L, et al.
Endocrinology, 148, 849?856-849?856 (2007)
Activation of G Protein-Coupled Receptor 43 in Adipocytes Leads to Inhibition of Lipolysis and Suppression of Plasma Free Fatty Acids
Hongfei G, et al.
Endocrinology, 149, 4519?4526-4519?4526 (2008)
Bidisha Dasgupta et al.
European journal of immunology, 38(12), 3499-3507 (2008-11-11)
When leukocytes cross endothelial cells during the inflammatory response, membrane from the recently described lateral border recycling compartment (LBRC) is selectively targeted around diapedesing leukocytes. This "targeted recycling" is critical for leukocyte transendothelial migration. Blocking homophilic PECAM interactions between leukocytes
N C Schaad et al.
Human reproduction (Oxford, England), 11(3), 561-565 (1996-03-01)
Nitric oxide is a chemical messenger which functions as a neurotransmitter or as a cytotoxic agent. Nitric oxide synthase (NOS) has been isolated from various mammalian reproductive tissues. The presence or absence of NOS in spermatozoa has not yet been
Guangrui Yang et al.
PloS one, 7(8), e38117-e38117 (2012-08-18)
Compelling evidence from both human and animal studies suggests a physiological link between the circadian rhythm and metabolism but the underlying mechanism is still incompletely understood. We examined the role of PPARγ, a key regulator of energy metabolism, in the
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Protocoles

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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