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Merck
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IP0010

Millipore

Anti-HA Immunoprecipitation Kit

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.56

Type de produit anticorps

primary antibodies

Niveau de qualité

200

Clone

monoclonal

Classe(s) chimique(s) de l'analyte

proteins (hemagglutinin)

Technique(s)

immunoprecipitation (IP): suitable

Température de stockage

2-8°C

Catégories apparentées

Description générale

Anti-hemagglutinin (HA) Immunoprecipitation Kit is designed to recover maximum amounts of HA-tagged proteins in the immunoprecipitates. The process is carried out in mini-spin columns instead of microcentrifuge tubes to ensure easy washing of antigen-antibody-bound beads with minimal variability.

Application

Anti- hemagglutinin (HA) Immunoprecipitation Kit has been used for immunoprecipitation. It has also been used in in vitro acetylation assay from human embryonic kidney cell lines.

Caractéristiques et avantages

  • Anti- hemagglutinin (HA) coupled beads enable high specific binding to HA-tagged fusion proteins
  • Coupling of Anti-HA antibodies to the beads eliminates other preliminary and calibration steps
  • Mini-spin columns enable the minimal loss of anti-HA-bound beads during washing
  • The CelLyticTM M cell lysis reagent provides a high yield, rapid and efficient mammalian cell lysis
  • Ready-to-use cell lysis reagent and 10X concentrated immunoprecipitation buffer

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Réf. du produit
Description
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  • A2095Monoclonal Anti-HA−Agarose antibody produced in mouse, clone HA-7, purified immunoglobulin, PBS suspension .5 mLFDS
  • C2978CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 50 mLFDS

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Paola Krall et al.
PloS one, 5(9), e12859-e12859 (2010-09-30)
Mutations in the TRPC6 calcium channel (Transient receptor potential channel 6) gene have been associated with familiar forms of Focal and Segmental Glomerulosclerosis (FSGS) affecting children and adults. In addition, acquired glomerular diseases are associated with increased expression levels of
Lin Jin et al.
PLoS pathogens, 12(5), e1005609-e1005609 (2016-05-19)
Bacterial AvrE-family Type-III effector proteins (T3Es) contribute significantly to the virulence of plant-pathogenic species of Pseudomonas, Pantoea, Ralstonia, Erwinia, Dickeya and Pectobacterium, with hosts ranging from monocots to dicots. However, the mode of action of AvrE-family T3Es remains enigmatic, due
Satadru K Lahiri et al.
American journal of cancer research, 6(5), 910-923 (2016-06-14)
Krüppel-like factor 8 (KLF8) plays important roles in cancer and is strictly regulated by various post-translational modifications such as sumoylation, acetylation, ubiquitylation and PARylation. Here we report a novel phosphorylation of KLF8 by ERK2 responsible and critical for the stability
Xiaolong Tang et al.
Nature communications, 12(1), 5058-5058 (2021-08-27)
Dietary interventions such as intermittent fasting (IF) have emerged as an attractive strategy for cancer therapies; therefore, understanding the underlying molecular mechanisms is pivotal. Here, we find SIRT7 decline markedly attenuates the anti-tumor effect of IF. Mechanistically, AMP-activated protein kinase
Jian Sun et al.
The Journal of biological chemistry, 293(21), 8275-8284 (2018-04-08)
Connexin-43 (Cx43, also known as GJA1) is the most ubiquitously expressed connexin isoform in mammalian tissues. It forms intercellular gap junction (GJ) channels, enabling adjacent cells to communicate both electrically and metabolically. Cx43 is a short-lived protein which can be
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