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Key Documents

GE17-0010-01

Sephadex® G-10

Cytiva 17-0010-01, pack of 100 g

Synonyme(s) :

Sephadex G-10 column, Sephadex gel

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About This Item

Code UNSPSC :
23151817
Nomenclature NACRES :
NA.56

Caractéristiques

autoclavable In wet form (pH 7.0) at 120°C for 30 min

Conditionnement

pack of 100 g

Fabricant/nom de marque

Cytiva 17-0010-01

Paramètres

flow rate (The beads behave as rigid spheres obeying Darcy′s Law.)

Matrice

cross-linked dextran

Taille des particules

40-120 μm (dry)
55-165 (wet)

working range

2-13

Description générale

Sephadex® G-10 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules > 700 molecular weight. Smallest Exlusion limit in the Sephadex® range.

Application

Sephadex® is a gel filtration medium prepared by crosslinking Dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency fractionation with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and Low operating pressures are required.

Caractéristiques et avantages

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Suitable for buffer exchange and cleanup of biological samples, for example peptides, small Proteins, and oligosaccharides larger than 700 molecular weight
  • Efficient removal of contaminants such as salts, dyes, and radioactive labels
  • Lowest exclusion limit (700 molecular weight) in the Sephadex® G-range

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sephadex is a registered trademark of Cytiva

Code de la classe de stockage

13 - Non Combustible Solids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Protocoles

Performing a Separation with Sephadex®

This page shows how to perform a separation with a Sephadex column from Cytiva which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.

Column Packing and Preparation for Size Exclusion Chromatography

Column Packing and Preparation for Size Exclusion Chromatography

Contenu apparenté

Sephadex® and Darcy’s Law

Sephadex® from Cytiva and Darcy’s law in Size Exclusion Chromatography

Separation Options with Sephadex®

Separation options for Size Exclusion Chromatography with Sephadex from Cytiva.

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