DNAQF
DNA Quantitation Kit, Fluorescence Assay
Quantitation of DNA using bisBenzimide
Synonyme(s) :
dsDNA quantification
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About This Item
Code UNSPSC :
41105500
Nomenclature NACRES :
NA.84
Produits recommandés
Niveau de qualité
Utilisation
sufficient for 750 assays (2 ml each)
Technique(s)
nucleic acid detection: suitable
Température de stockage
−20°C
Description générale
The kit has all the reagents necessary for quantitation of DNA using the fluorescent dye bisBenzimide (Hoechst 33258). The kit allows highly sensitive, quick, and accurate determinations of double-stranded DNA from 10ng/ml to 5μg/ml, even in the presence of RNA and proteins. Fluorometry is a highly sensitive and simple method used for DNA quantitation. The fluorescent dye, bisBenzimide H 33258, which binds primarily to AT sequences in the minor groove of double-stranded DNA (dsDNA), is specific for quantitation of nanogram amounts of DNA (10 ng/ml to 10 mg/ml). When excited at 360 nm, the fluorescence emission at 460 nm of the dye increases significantly in the presence of DNA.
Application
DNA Quantitation Kit, Fluorescence Assay has been used to measure the concentration/content of DNA:
- in neonatal rat ventricular myocytes (NRVMs)
- in human mesenchymal stem cells (hMSCs)/samples for normalization of the relative alkaline phosphatase (ALP) amount
- in mouse embryonic fibroblast cell line (NIH3T3 cells)
Caractéristiques et avantages
- Specific for the quantitation of nanogram amounts of DNA
- Works well with purified preparations as well as with DNA from crude extracts containing RNA and proteins
Composants de kit seuls
Réf. du produit
Description
- Detailed technical bulletin 1 ea
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
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