D6434

DMEM/F-12

With HEPES, sodium bicarbonate and sodium pyruvate, without ʟ-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

• Synonyme(s) : DME/F-12, mélange 1/1
• UNSPSC Code: 12352207
• NACRES: NA.75
• MDL number: MFCD00217417

Propriétés

• Nom du produit: Milieu de Eagle modifié par Dulbecco (DMEM)/Mélange nutritif F-12 de Ham, With 15 mM HEPES and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
• Quality Segment: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: L-glutamine: no; phenol red: no; NaHCO₃: yes; HEPES: 15 mM; sodium pyruvate: 0.055 g/L; glucose: 3.15 g/L
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. Suitable for growth of a wide range of cell types in low serum conditions. Absence of phenol red makes this a preferred medium for clonal and stem cell culture.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture:

• bovine endometrial epithelial cells
• cancer stem cells
• breast cancer cell line

Preparation Note

Supplement with 0.365 g/L L-glutamine

Informations sur la sécurité

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Classe de stockage: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable