h1RPE7

9061602, human eye, Polygonal

• Code UNSPSC : 41106514

Propriétés

• product name: h1RPE7, 09061602, from human eye
• Source biologique: human eye
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Polygonal
• Produits: Cytokeratins 5 and 8, junctional protein zonula occludens (ZO-1)
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice

Description

Origine de la lignée cellulaire

An immortalized retinal pigment epithelial cell line

Description de la lignée cellulaire

The h1RPE7 cell line was derived from primary human retinal pigment epithelial cells obtained from a 50-year-old female Caucasian donor. The primary culture was transfected with a construct encoding SV40 large T antigen that was deleted for the small t antigen along with puromycin resistance gene.

Profil d'ADN

STR-PCR Data:
Amelogenin: X
CSF1PO: 10,13
D13S317: 12
D16S539: 12
D5S818: 9,13
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 16,19

Milieu de culture

Hams F10 + 20% Heat-inactivated FCS + 2 mM Glutamine + selection antibiotic 1μg/ml puromycin.

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approx 24hrs.

Autres remarques

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Clause de non-responsabilité

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.