C-12511

Human Coronary Artery Smooth Muscle Cells (HCASMC)

500,000 cryopreserved cells

• Synonyme(s) : Human smooth muscle cells
• Code UNSPSC : 41106514
• Nomenclature NACRES : NA.81

Propriétés

• Source biologique: human coronary artery
• Conditionnement: pkg of 500,000 cells
• Morphologie: (smooth muscle)
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): hypertension; cardiovascular diseases
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description générale

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Origine de la lignée cellulaire

Artery

Application

Primary Human Coronary Artery Smooth Muscle Cells (HCASMC) are isolated from human coronary arteries and are provided in a cryopreserved format. HCASMC stain positive for smooth muscle α-actin. Coronary arteries supply the heart with blood and obstruction in atherosclerosis causes myocardial infarction.HCASMC are qualified to study the function of smooth muscle cells in the development of atherosclerotic plaques or in other diseases like hypertension. They are also useful for stent-graft compatibility testing.

Qualité

Rigid quality control tests are performed for each lot of Human Smooth Muscle Cells. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. smooth muscle a-actin, are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Avertissement

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Procédure de repiquage

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Autres remarques

Recommended Plating Density: 7500 - 10000 cells per cm²Passage After Thawing: P2Tested Markers: Smooth muscle alpha-actin positiveGuaranteed population doublings: >15

Produits recommandés

Recommended Primary Cell Culture Media:Link

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du Ministère français de la Recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable