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A3699

Sigma-Aldrich

Acrylamide/Bis-acrylamide solution

Acrylamide/Bis-acrylamide, 30% solution

30%, BioReagent, suitable for electrophoresis, 37.5:1

Synonyme(s) :

30% Acrylamide/Bisacrylamide 37.5:1, Polyacrylamide solution

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About This Item

Numéro MDL:
MFCD00080848
Code UNSPSC :
41105319
ID de substance PubChem :
329770843
Nomenclature NACRES :
NA.25
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Nom du produit

Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 37.5:1

Stérilité

0.2 μm filtered

Niveau de qualité

100

Gamme de produits

BioReagent

Forme

liquid

Ratio alimentaire

37.5:1

Technique(s)

electrophoresis: suitable

Adéquation

suitable for electrophoresis

Température de stockage

2-8°C
2-8°C

Chaîne SMILES 

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

Clé InChI

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

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Description générale

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent.[1] On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.[2]

Application

Acrylamide/Bis-acrylamide, 30% solution has been used:
  • in the preparation of polyacrylamide gel for photobleaching experiment[3]
  • as a constituent in the preparation of gel precursor solution and pore-gradient microgel precursor solution[4]
  • in the preparation of polyacrylamide gels during western blot analyses[5]

These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Notes préparatoires

Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Organes cibles

Peripheral nervous system

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificats d'analyse (COA)

Lot/Batch Number
0000340944
SLCQ1813
SLCK8593
SLCF0473
SLCB5712

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Ana Techniqs in Biotechnology (2011)
Single cell--resolution western blotting
Kang CC, et al.
Nature Protocols, 11(8), 1508-1508 (2016)
Light-dependent degradation of PIF3 by SCFEBF1/2 promotes a photomorphogenic response in Arabidopsis
Dong J, et al.
Current Biology, 27(16), 2420-2430 (2017)
Engineering and characterizing monomeric fluorescent proteins for live-cell imaging applications
Ai HW, et al.
Nature Protocols, 9(4), 910-910 (2014)
Qiong Pan et al.
Analytica chimica acta, 1000, 214-222 (2018-01-01)
Arrays of microwells are widely used to isolate individual cells, facilitate high throughput cytometry assays, and ensure compatibility of those assays with whole-cell imaging. Microwell geometries have recently been utilized for handling and preparation of single-cell lysate, prior to single-cell
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Questions

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  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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