A3429
Differentiation Solution
for the differentiation of regressive hematoxylin stains
Synonyme(s) :
Acid Alcohol
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About This Item
Produits recommandés
Forme
solution
Durée de conservation
40 mo.
Couleur
colorless
Application(s)
hematology
histology
Température de stockage
room temp
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Description générale
Differentiation solution comprises acidified ethanol or isopropanol that is used to decolorize hematoxylin stain. The duration of exposure to the solution determines the degree of decolorization. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper color contrast necessary for visualization, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.
Application
Differentiation solution is employed mainly for the differentiation of regressive hematoxylin stains. It has been used in the following studies:
- the prediction of pulmonary metastasis progression in osteosarcoma patients
- the development of multispectral imaging to detect melanin
Acidified alcohol solution for the differentiation of regressive hematoxylin stains.
Caractéristiques et avantages
- Desired staining patterns can be obtained by varying the degree of exposure to the differentiation solution.
- Results are sharper and more rapid compared to other differentiation methods.
- Crisp and clear differentiation between cellular structures is obtainable when performed according to Sigma-Aldrich Procedure No. GHS.
Principe
In regressive hematoxylin staining, a highly concentrated hematoxylin solution is used to rapidly overstain both nuclei and cytoplasm, and the cytoplasm is subsequently decolorized and excess hematoxylin is removed from the nucleus with dilute acid. Improper differentiation leaves excess residual hematoxylin that obscures the fine details of nuclear structures and chromatin and prevents eosin uptake.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - STOT SE 2
Organes cibles
Eyes,Central nervous system
Code de la classe de stockage
3 - Flammable liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
71.6 °F
Point d'éclair (°C)
22 °C
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Nuclear staining with alum hematoxylin
B D Llewellyn
Biotechnic & Histochemistry, 84(9), 159-177 (2009)
Multispectral imaging as a tool for melanin detection
Kalleberg, et al.
Journal of Histotechnology, 38(1), 14-21 (2015)
Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root
Andres-Manzano, et al.
Methods in Molecular Biology, 1339 (2015)
Pia M Teleman et al.
Acta obstetricia et gynecologica Scandinavica, 88(8), 927-932 (2009-07-07)
To outline possible associations between urinary incontinence (UI) and serum levels of steroid hormones in middle-aged women. Community-based observational study. All women aged 50-59 living in the Lund area by December 1995 were invited to a screening procedure. Sixty-four percent
M Jesús Andrés-Manzano et al.
Methods in molecular biology (Clifton, N.J.), 1339, 85-99 (2015-10-09)
Methods for staining tissues with Oil Red O and hematoxylin-eosin are classical histological techniques that are widely used to quantify atherosclerotic burden in mouse tissues because of their ease of use, reliability, and the large amount of information they provide.
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