T3163

Tetramethylrhodamine isothiocyanate Isomer R

powder

• Synonyme(s) : 6-TRITC, Tetramethylrhodamine-6-isothiocyanate
• Formule empirique (notation de Hill): C₂₅H₂₁N₃O₃S
• Numéro CAS: 80724-20-5
• Poids moléculaire : 443.52
• Numéro MDL: MFCD00063517
• Code UNSPSC : 12171500
• ID de substance PubChem : 24900112
• Nomenclature NACRES : NA.47

Propriétés

• Description: labeling efficiency with bovine albumin >= 50%
• Niveau de qualité: 200
• Forme: powder
• Composition: Dye content, ≥80%
• Solubilité: 1 M NH4OH: 10 mg/mL (Faint Red to Very Dark Red to Very Dark Purple solution)
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: 2-8°C
• Chaîne SMILES: CN(C)c1ccc2c(OC3=C\C(C=CC3=C2c4cc(ccc4C([O-])=O)N=C=S)=[N+](/C)C)c1
• InChI: 1S/C25H21N3O3S/c1-27(2)16-6-9-19-22(12-16)31-23-13-17(28(3)4)7-10-20(23)24(19)21-11-15(26-14-32)5-8-18(21)25(29)30/h5-13H,1-4H3
• Clé InChI: OBYNJKLOYWCXEP-UHFFFAOYSA-N

Description

Description générale

Tetramethylrhodamine isothiocyanate Isomer R (TRITC) is the R isomer of tetramethylrhodamine isothiocyanate and is also known as 6-tetramethylrhodamine isothiocyanate. It is a strongly fluorescent compound soluble in ethanol, methanol, dimethylformamide, dimethylsulfoxide, and water. TRITC is a moderately-sized aminoxanthene dye carrying carboxylic acid and isothiocyanate substituents on the non-planar pendant phenyl ring. It forms a lipophilic cation in acidic pH and a hydrophilic zwitterion around neutral pH. The isothiocyanate group reacts with nucleophilic substituents (e.g., amino, hydroxyl, thiol) of biomolecules, providing the means of attaching a fluorescent label. TRITC also reacts with water and hydroxide ions and is unstable in aqueous solutions. TRITC is commercially available as a zwitterion or as a chloride.

Application

• TRITC is used to label a wide variety of biomolecules, including immunoglobulins, lectins, nucleic acids, polynucleotides, and polysaccharides for affinity, immuno-, and in situ hybridization, fluorescent probes, and flow cytometry.
• Lectins are TRITC-Iabeled reagents for the affinity staining of sections and cell monolayers to distinguish the sialomucins of salivary glands.
• TRITC-antibodies are used to identify pathogenic amoebae.
• TRITC-oligonucleotides are used in the in-situ hybridization staining of soil microorganisms.
• TRITC-Iabeled reagents have also been used as fluorescent probes of live cells.
• Flow cytometry of TRlTC-Iabeled slime mold cells has been used to study their aggregation behavior.

Informations sur la sécurité

• Mentions de danger: H412
• Conseils de prudence: P273
• Classification des risques: Aquatic Chronic 3
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable