MABF2674

Anti-IL-18 Antibody

purified antibody, clone 11–4.1, from mouse

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 11–4.1, monoclonal
• Poids mol.: calculated mol wt 22.33 kDa; observed mol wt ~19 kDa
• Espèces réactives: human
• Conditionnement: antibody small pack of 100 μL
• Technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG
• Conditions d'expédition: dry ice
• Température de stockage: -10 to -25°C
• Modification post-traductionnelle de la cible: unmodified

Description

Spécificité

Clone 11-4.1 is a mouse monoclonal antibody that detects human IL-18 protein. It targets an epitope within 6 amino acids from the N-terminal half.

Immunogène

His-tagged full-length active recombinant human Interleukin-18 (without the propeptide).

Application

Quality Control TestingEvaluated by Western Blotting in HeLa cell lysate.Western Blotting Analysis: A 1:500 dilution of this antibody detected IL-18 in HeLa cell lysate.Tested ApplicationsImmunoprecipitation Analysis: A representative lot immunoprecipitated IL-18 in Immunoprecipitation applications (Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).Immunocytochemistry Analysis: A representative lot detected IL-18 in Immunocytochemistry applications (Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).Western Blotting Analysis: A representative lot detected IL-18 in Western Blotting applications (Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected IL-18 in HeLa cells.Inhibition Assay: A representative lot inhibited IL-18 involved interferon release from KG-1 cells.(Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82). Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-IL-18, clone 11 4.1, Cat. No. MABF2674, is a mouse monoclonal antibody that detects Interleukin-18 and is tested for use in Immunocytochemistry, Immunoprecipitation, Inhibition Assay, and Western Blotting.

Description de la cible

Interleukin-18 (UniProt: Q14116; also known as IL-18, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma) is encoded by the IL18 (also known as IGIF, IL1F4) gene (Gene ID: 3606) in human. IL-18 is a pro-inflammatory cytokine that is shown to evoke both innate and acquired responses. It is synthesized with a propeptide (aa 1-36), which is subsequently cleaved off by caspases 1 and 4 to generate the active mature form. IL-18 is reported to be constitutively expressed in monocytes, macrophages, epithelial cells, osteoblasts, keratinocytes, and synovial fibroblasts. Its expression has also been reported in adrenal cortical cells. Mature IL-18 acts by binding to the IL-18 receptor a (IL-18a) to form a low affinity binary complex that recruits IL-18b to form a high affinity complex that leads to the activation of NF-kB and MAP kinase pathways. IL-18 can synergize with IL-12 to induce interferon  synthesis from T-helper 1 and natural killer cells. Clone 11-4.1 is shown to block IL-18 evolved interferon release in a dose-dependent manner (IC50 = 14.2 nM). (Ref.: Nariai, Y., et al. (2019). Arch. Biochem. Biophys. 663; 71-82).

Forme physique

Purified mouse monoclonal antibody IgG2b in PBS without preservatives.

Stockage et stabilité

Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable