ABD125

Anti-TFE3 Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : TFE3, Transcription factor E3, Class E basic helix-loop-helix protein 33, bHLHe33
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_006512
• Numéro d'accès UniProt: P19532
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TFE3(7030)

Description

Description générale

The DNA-binding factor TFE3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. TFE3 is ubiquitously expressed and can directly associates with DNA as either homodimers or heterodimers formed with two related proteins, TFEB or TFEC. TFE3 binds to and activates the microE3 motif of the immunoglobulin heavy-chain enhancer to induce B-cell-specific gene transcription and DNA recombination. TFEB is expressed at low levels in the embryo but at high levels in the trophoblast cells of the placenta, where it plays a critical role in regulating normal vascularization of the placenta. Recently, Smith et al. have proven that the exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor TFE3 (Cell 153, 335–347, April 11, 2013).

Immunogène

linear peptide corresponding to Human TFE3.

Application

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory on TFE3 overexpressing cell lines, epiblast and primitive endodermal cells (Smith, A., et al. (2013). Cell. 153, 335–347).

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

This Anti-TFE3 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of TFE3.

Qualité

Evaluated by Western Blot in a HEK293T cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected TFE3 in 10 µg of HEK293T cell lysate.
Uncharacterized band(s) may appear in some lysates with longer exposure times.

Description de la cible

~ 65 kDa observed

Forme physique

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS, 50% glycerol, and 0.05% sodium azide

Stockage et stabilité

Store at -20°C

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2