AB3403-I

Anti-Beta Galactosidase Antibody, E. coli

from chicken, purified by affinity chromatography

• Synonyme(s) : Beta-galactosidase, Acid beta-galactosidase, Lactase, Elastin receptor 1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: chicken
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: bacteria
• Technique(s): western blot: suitable
• Numéro d'accès NCBI: NP_752394
• Numéro d'accès UniProt: P16278
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: Escherichia coli K12 ... Lacz(945006)

Description

Description générale

β Galactosidase, also known as Beta-galactosidase, Beta-gal, LacZ, or Lactase, and encoded by the gene lacZ, is the enzyme in bacteria that hydrolyzes galactosides (sugars chains containing galactose) in to monosaccharides. β Galactosidase is typically an extracellularly produced enzyme that hydrolyzes the beta-glycosidic bond between galactose and another organic moiety. In E. coli, the β Galactosidase gene is present as part of the inducible lac operon system which is activated in the presence of lactose when glucose level is low. Today, bacterial beta galactosidase is used in molecular biology as a reporter marker to monitor gene expression, and because the enzyme has two functional domains, it forms the basis for α-complementation which is the methodology behind for the blue/white screening of recombinant clones. The presence or absence of an active β-galactosidase may be detected by X-gal, which produces a characteristic blue dye when cleaved by β-galactosidase, thereby providing an easy means of distinguishing the presence or absence of cloned product in a plasmid.

Immunogène

Recombinant protein corresponding to E. Coli Beta Galactosidase.

Application

Anti-Beta Galactosidase Antibody, E. coli is a highly specific chicken polyclonal antibody & that targets β-Galactosidase & has been tested in western blotting.

Qualité

Evaluated by Western Blotting in TOP10/PCU19 E. Coli lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Beta Galactosidase in 10 µg TOP10/PCU19 E. Coli lysate.

Description de la cible

~120 kDa observed

Liaison

Replaces: AB3403

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable