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567704

Sigma-Aldrich

Sphingolipid Ceramide N-Deacylase, Pseudomonas sp.

Synonyme(s) :

SCDase

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

Source biologique

bacterial (Pseudomonas spp.)

Niveau de qualité

100

Forme

liquid

Activité spécifique

≥1 units/mL

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles

Activité étrangère

α- and β-N-acetylgalactosaminidase, α-fucosidase, α- and β-galactosidase, neuraminidases, none detected

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

Sphingolipid ceramide N-deacylase (SCDase) is a native sphingolipid ceramide N-deacylase from Pseudomonas species. It hydrolyzes the N-acyl linkage between fatty acids and the sphingosine base of ceramide in several sphingolipids and gangliosides. This enzyme catalyzes the condensation reaction between fatty acids and sphingosine bases and forms sphingolipids. It is also involved in transacylation reactions. SCDase is not recommended for hydrolysis of ceramide. It is also used to prepare lysoglycosphingolipids (lysoGSLs) effortlessly.

Application

Sphingolipid ceramide N-Deacylase, Pseudomonas sp. has been used as a PP2A enhancer to study its effects on phosphatase and tensin homolog (PTEN) in forkhead box O3 (FOXO3a) phosphorylation in primary CD4+ T cells.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of asialo-GM₁ per min at 37°C, pH 6.0.

Forme physique

In 50 mM sodium acetate buffer, 0.1% LUBROL PX Detergent, pH 6.0.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Autres remarques

Mitsutake, S., et al. 1997. Anal. Biochem. 247, 52.
Sueyoshi, N., et al. 1997. J. Lipid Res. 38, 1923.
Ito, M., et al. 1995. J. Biol. Chem. 270, 24370.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Nayoung Kim et al.
PLoS pathogens, 6(9), e1001103-e1001103 (2010-09-24)
Apoptosis in HIV-1-infected CD4+ primary T cells is triggered by the alteration of the PI3K and p53 pathways, which converge on the FOXO3a transcriptional activator. Tat alone can cause activation of FOXO3a and of its proapoptotic target genes. To understand
S Mitsutake et al.
Analytical biochemistry, 247(1), 52-57 (1997-04-05)
Sphingolipid ceramide N-deacylase (SCDase) is an enzyme which hydrolyzes the N-acyl linkage between fatty acid and sphingosine in ceramide of various glycosphingolipids and sphingomyelin. Recently the enzyme was found to catalyze the hydrolysis and its reverse reaction under different conditions.
T Kurita et al.
Journal of lipid research, 41(5), 846-851 (2000-04-29)
Lysoglycosphingolipids were produced from glycosphingolipids by using sphingolipid ceramide N-deacylase, which cleaves the N-acyl linkage between fatty acids and sphingosine bases in various glycosphingolipids. The enzyme reaction was done in a biphasic media prepared with water;-immiscible organic solvent and aqueous

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