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411492

Sigma-Aldrich

Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6025)

liquid, clone HP6025, Calbiochem®

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

secondary antibodies

Clone

HP6025, monoclonal

Forme

liquid

Ne contient pas

preservative

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles

Isotype

IgG

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Purified mouse monoclonal antibody. Recognizes human IgG4 Fc fragment.
This Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6025) is validated for use in Enzyme Immunoassay for the detection of Human IgG₄, Fc Fragment Specific.

Application

Enzyme Immunoassay (10 µg/ml, see application references)

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In PBS, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.

Autres remarques

Antibody should be titrated for optimal results in individual systems.



Cross-reactivity by ELISA against human myeloma proteins:

Human IgG1: ≤0.1%

Human IgG2: ≤0.04%

Human IgG3: ≤0.02%

Human IgG4: 100%

Human IgA: <0.1%

Human IgE: <0.1%

Human IgM: <0.1%



Cross-reactivity by ELISA against chimeric antibodies:

Human IgG1: <0.01%

Human IgG2: <0.01%

Human IgG3: <0.01%

Human IgG4: 100%

Human IgA: <0.01%

Human IgE: <0.01%

Human IgM: <0.01%
Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

D C Houpt et al.
Infection and immunity, 62(7), 2857-2864 (1994-07-01)
Serum obtained from normal human subjects contains antibodies reactive in an enzyme-linked immunosorbent assay with the glucuronoxylomannan (GXM) of Cryptococcus neoformans. The frequency of occurrence of class-specific antibodies among normal subjects was 28% for immunoglobulin G (IgG), 98% for IgM
R G Keller et al.
Infection and immunity, 62(1), 215-220 (1994-01-01)
Previous studies found that normal human serum (NHS) contains an immunoglobulin G (IgG) antibody that mediates initiation of the classical complement pathway by nonencapsulated Cryptococcus neoformans. The present study used an enzyme-linked immunosorbent assay with whole nonencapsulated yeast cells as
S Sievers et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 47(8), 1057-1068 (2017-03-28)
Microarray-based component-resolved diagnostics (CRD) has become an accepted tool to detect allergen-specific IgE sensitization towards hundreds of allergens in parallel from one drop of serum. Nevertheless, specificity and sensitivity as well as a simultaneous detection of allergen-specific IgG4 , as
H Lu et al.
Infection and immunity, 61(6), 2400-2407 (1993-06-01)
The aims of this study were to determine the immunodominant antigens of Actinobacillus actinomycetemcomitans serotype b (Aab) for the different immunoglobulin (Ig) classes and subclasses and to determine the relative levels of these different Igs in serum. Seropositive early-onset periodontitis

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