1.11608

Ethidium bromide

(1% solution in water) for electrophoresis

• Code UNSPSC : 41105305
• Nomenclature NACRES : NA.51

Propriétés

• Niveau de qualité: 100
• Forme: liquid
• pH: 4.4 (20 °C in H₂O)
• Densité: 1.00 g/cm³ at 20 °C
• Température de stockage: 2-8°C

Description

Application

Ethidium bromide has been used to stain polyacrylamide gels for small-scale transcription reactions. It has also been used for the quantification of DNA.

Actions biochimiques/physiologiques

Ethidium bromide is a red fluorescent dye that reacts with the DNA of membrane-damaged cells by intercalating. It is used for visualizing DNA in gels. Ethidium bromide is carcinogenic with moderate toxicity.

Caractéristiques et avantages

Robust and easy to use

Remarque sur l'analyse

Identity (UV/VIS-Spectrum): passes test
Absorption maximum (water): 478 - 482 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.004 %; water): 140 - 160
Suitability for electrophoresis: passes test

Informations sur la sécurité

• Pictogrammes: GHS06,GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H331 - H341
• Conseils de prudence: P202 - P261 - P271 - P280 - P304 + P340 + P311 - P308 + P313
• Classification des risques: Acute Tox. 3 Inhalation - Muta. 2
• Code de la classe de stockage: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable