1.01597

AFB-Fluor phenol-free

Staining kit for the examination of acid-fast bacteria with fluorescence microscopy (Auramin-Rhodamine staining)

• Code UNSPSC : 41116130
• Nomenclature NACRES : NA.74

Propriétés

• Niveau de qualité: 400
• IVD: for in vitro diagnostic use
• Application(s): clinical testing; diagnostic assay manufacturing; hematology; histology
• Méthode de détection: fluorometric (microscopy)
• Température de stockage: 15-25°C

Description

Description générale

The decisive feature of the AFB-Fluor phenol-free for detection of aldehyde and mucosubstances, is the presence of fluorescence dyes in the staining solution. This enables the swift and secure identification of the acid-fast bacteria under the fluorescence microscope. As it has become an established procedure alongside Ziehl-Neelson staining (AFB-Color modified), we provide three ready-to-use solutions in our AFB-fluor, phenol-free staining kit. Of these, the first solution contains the two fluorescent dyes, Rhodamine B – staining red - and Auramine O – staining yellowish-green – which can be used depending on the filter combination of the microscope. Here, the auramine-rhodamine staining solution is prepared without phenol and the efficient penetration of the dyes into the target structures is achieved through a phenol substitute that is more user- and eco-friendly than phenol. Further, the kit supplies a decolorization solution and a potassium permanganate solution that turns the background uniformly black, suppressing troublesome intrinsic fluorescence of the specimen. If required, double staining with AFB-Color modified (Product number 1.00497) (ZN) is possible. The AFB-fluor, phenol-free-kit, modified is designed to be used for smears, thougt it can also be used for paraffin sections.
The kit is sufficient for up to 60-65 preparations and is an IVD / CE registered product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Remarque sur l'analyse

Suitability for microscopy: passes test
Mycobacteria: red-orange/yellow-green
Background: dark

Informations sur la sécurité

• Pictogrammes: GHS02,GHS05,GHS07,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H225 - H290 - H319 - H336 - H411
• Conseils de prudence: P210 - P233 - P234 - P240 - P273 - P305 + P351 + P338
• Classification des risques: Aquatic Chronic 2 - Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 3 - Flammable liquids
• Classe de danger pour l'eau (WGK): WGK 3