06-1297

Anti-U1-70K Antibody

from rabbit

• Synonyme(s) : small nuclear ribonucleoprotein 70kDa (U1), U1 small nuclear ribonucleoprotein 70 kDa, small nuclear ribonucleoprotein 70kDa (RNP antigen), U1 snRNP 70 kDa
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: mouse, human
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_003080.2
• Numéro d'accès UniProt: P08621
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SNRNP70(6625)

Description

Description générale

U1 small nuclear ribonucleoprotein 70 kDa (U1-70K) is one of three specific proteins found within the U1 snRNP core, which includes at least seven core proteins found in all U snRNPs and three specific proteins; U1-70K, U1-A, and U1-C. U1-70K is involved in mediating pre-mRNA splicing through binding the loop I region of U1-snRNA with its RPM (RNA recognition motif) binding domain. In addition, its RNA binding domain, U1-70K also contains two argine-rich regions, accented with acidic or serine residues, that is thought to have trans-acting functions. Alternative splicing results in 4 known isoforms. Truncated forms of U1-70K are unable to bind RNA.

Spécificité

This antibody recognizes U1-70K.

Immunogène

Recombinant protein corresponding to C-terminal domain of human U1-70K.

Application

Anti-U1-70K Antibody is a Rabbit Polyclonal Antibody for detection of U1-70K also known as small nuclear ribonucleoprotein 70kDa (U1) & has been validated in WB, ICC.

Immunocytochemistry Analysis: 1:500 of a representative lot of this antibody has been shown to detect U1-70K in NIH/3T3, A431, and HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Qualité

Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected U1-70K on 10 µg of HepG2 cell lysate.

Description de la cible

Calculated 51 kDa Observed ~57 kDa

Forme physique

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
HepG2 cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable