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05-613

Sigma-Aldrich

Anti-β-Catenin Antibody, clone 2H4A7

clone 2H4A7, Upstate®, from mouse

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

2H4A7, monoclonal

Espèces réactives

rat, mouse, human

Fabricant/nom de marque

Upstate®

Technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

mouse ... Ctnnb1(12387)

Spécificité

β-Catenin

Immunogène

fusion protein corresponding to residues 1-100 of human β-Catenin

Application

Detect β-Catenin also known as beta catenin with Anti-β-Catenin Antibody, clone 2H4A7 (Mouse Monoclonal Antibody), that has been demonstrated to work in IP, WB & IHC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Qualité

routinely evaluated by immunoblot on RIPA lysates from A431, mouse 3T3/A31 or rat L6 cell lysates

Description de la cible

92kDa

Liaison

Replaces: 04-1002

Forme physique

Format: Purified
Protein G Chromatography
Protein G purified IgG in buffer containing 0.1M Tris-Glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide

Stockage et stabilité

Stable 2 years at 2-8°C

Remarque sur l'analyse

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1


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Consulter la Bibliothèque de documents

Josiane Sancéau et al.
The Journal of biological chemistry, 278(38), 36537-36546 (2003-07-09)
Enhanced expression of (pro)matrix metalloproteinase-9 (MMP-9) is associated with human tumor invasion and/or metastasis. COH cells derived from a highly invasive and metastatic Ewing's sarcoma constitutively express proMMP-9. Transfection of a double stranded RNA that targets the MMP-9 mRNA into
A Bowen et al.
Biochemical and biophysical research communications, 479(4), 793-799 (2016-10-01)
The Wnt signalling pathway in beta-cells has been linked to the development of type 2 diabetes. Investigating the impact of a non-canonical Wnt ligand, Wnt4, on beta-cell function we found that in INS-1 cells, Wnt4 was able to completely block Wnt3a
Ayako Mimata et al.
Cancer science, 102(5), 942-950 (2011-02-01)
Alterations in the E-cadherin gene are associated with sporadic and hereditary diffuse-type gastric cancer. To determine how the loss of function of E-cadherin affects gastric epithelial cell phenotypes, we generated transgenic mice using the Cre-loxP system in which the E-cadherin
Yu-Jen Chiu et al.
International journal of oncology, 52(5), 1465-1478 (2018-03-24)
Osteosarcoma is the most common primary malignancy of the bone and is characterized by local invasion and distant metastasis. Over the past 20 years, long-term outcomes have reached a plateau even with aggressive therapy. Overexpression of insulin-like growth factor 1 receptor
Nandaraj Taye et al.
Matrix biology : journal of the International Society for Matrix Biology, 120, 24-42 (2023-05-16)
Myogenesis is the process that generates multinucleated contractile myofibers from muscle stem cells during skeletal muscle development and regeneration. Myogenesis is governed by myogenic regulatory transcription factors, including MYOD1. Here, we identified the secreted matricellular protein ADAMTS-like 2 (ADAMTSL2) as

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