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790310P

Avanti

Transfection Reagent I

Avanti Research™ - A Croda Brand 790310P, powder

Synonyme(s) :

Transfection Reagent I, Transfection Reagent I, contains DOTAP:DOPE (1:1 w/w)

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About This Item

Code UNSPSC :
12352211
Nomenclature NACRES :
NA.25

Forme

powder

Conditionnement

pkg of 1 × 10 mg (790310P-10mg)
pkg of 2 × 100 mg (790310P-200mg)
pkg of 5 × 100 mg (790310P-500mg)
pkg of 1 × 25 mg (790310P-25mg)

Fabricant/nom de marque

Avanti Research™ - A Croda Brand 790310P

Application(s)

advanced drug delivery

Type de lipide

cationic lipids
transfection

Conditions d'expédition

dry ice

Température de stockage

−20°C

Catégories apparentées

Description générale

Transfection Reagent I comprises the cationic lipid 1,2-dioleoyloxypropyl)-N,N,N-trimethylammonium chloride (DOTAP) and the neutral lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine. DOTAP is a surfactant with double chain quaternary ammonium. The DOTAP/DOPE liposome takes lamellar and inverted hexagonal structures.

Application

Transfection Reagent I has been used in the preparation of liposomes along with cholesterol for fluorescence-based live-cell imaging studies in the mouse embryonic fibroblasts cells. It may be used for the transfection of DNAzyme (Dz13) into veins.

Actions biochimiques/physiologiques

Transfection Reagent I is used in large unilamellar cationic vesicles (LUVs) preparation and lipoplex generation. It improves transfection efficiency. DOTAP/DOPE (1,2-dioleoyloxypropyl)-N,N,N-trimethylammonium chloride /(1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) is safe transfection agent for human administration. It inhibits the blood vessel thickening and is a promising candidate for use in coronary artery bypass grafting (CABG).

Conditionnement

5 mL Clear Glass Sealed Ampule (790310P-10mg)
5 mL Clear Glass Sealed Ampule (790310P-200mg)
5 mL Clear Glass Sealed Ampule (790310P-25mg)
5 mL Clear Glass Sealed Ampule (790310P-500mg)

Informations légales

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Code de la classe de stockage

11 - Combustible Solids

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available

Certificats d'analyse (COA)

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Inhibition of vein graft stenosis with a c-jun targeting DNAzyme in a cationic liposomal formulation containing 1, 2-dioleoyl-3-trimethylammonium propane (DOTAP)/1, 2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE)
Li Y, et al.
International Journal of Cardiology, 168(4), 3659-3664 (2013)
Yue Li et al.
International journal of cardiology, 168(4), 3659-3664 (2013-07-28)
Coronary artery bypass grafting (CABG) is among the most commonly performed heart surgical procedures. Saphenous vein graft failure due to stenosis impedes the longer-term success of CABG. A key cellular event in the process of vein graft stenosis is smooth
Autophagy and formation of tubulovesicular autophagosomes provide a barrier against nonviral gene delivery
Roberts R, et al.
Autophagy, 9(5), 667-682 (2013)
Bieong-Kil Kim et al.
Biochimica et biophysica acta, 1848(10 Pt A), 1996-2001 (2015-06-27)
The effects of lipid compositions on their physicochemical properties and transfection efficiencies were investigated. Four liposome formulations with different 1,2-dioleoyl-3-trimethylammoniumpropane (DOTAP) to dioleoylphosphatidylethanolamine (DOPE) weight ratios were investigated, that is, weight ratios 1:0 (T1P0), 3:1 (T3P1), 1:1 (T1P1), and 1:3
Daniele Pezzoli et al.
Methods in molecular biology (Clifton, N.J.), 1025, 269-279 (2013-08-07)
Efficient delivery of nucleic acids into cells is a promising technique to modulate cellular gene expression for therapeutic and research applications. Cationic lipid-based liposomes represent one of the most intensively studied and employed nonviral vectors. They are positively charged at
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