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  • Anti-inflammatory effects of Siegesbeckia orientalis ethanol extract in in vitro and in vivo models.

Anti-inflammatory effects of Siegesbeckia orientalis ethanol extract in in vitro and in vivo models.

BioMed research international (2014-10-21)
Yong-Han Hong, Li-Wen Weng, Chi-Chang Chang, Hsia-Fen Hsu, Chao-Ping Wang, Shih-Wei Wang, Jer-Yiing Houng
ABSTRACT

This study aims to investigate the anti-inflammatory responses and mechanisms of Siegesbeckia orientalis ethanol extract (SOE). In cell culture experiments, RAW264.7 cells were pretreated with SOE and stimulated with lipopolysaccharide (LPS) for inflammatory mediators assay. In animal experiments, mice were tube-fed with SOE for 1 week, and s.c. injected with λ-carrageenan or i.p. injected with LPS to simulate inflammation. The degree of paw edema was assessed, and cytokine profile in sera and mouse survival were recorded. Data showed that SOE significantly reduced NO, IL-6, and TNF-α production in LPS-stimulated RAW264.7 cells. In vivo studies demonstrated that mice supplemented with 32 mg SOE/kg BW/day significantly lowered sera IL-6 level and resulted a higher survival rate compared to the control group (P = 0.019). Furthermore, SOE inhibited LPS-induced NF-κB activation by blocking the degradation of IκB-α. The SOE also reduced significantly the phosphorylation of ERK1/2, p38, and JNK in a dose-dependent manner. In summary, the in vitro and in vivo evidence indicate that SOE can attenuate acute inflammation by inhibiting inflammatory mediators via suppression of MAPKs- and NF-κB-dependent pathways.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ammonium pyrrolidinedithiocarbamate, purum p.a., ≥98.0% (NT)
Sigma-Aldrich
Ethyl palmitate, ≥95%, FG
Sigma-Aldrich
Ammonium pyrrolidinedithiocarbamate, ~99%
Sigma-Aldrich
Ethyl palmitate, ≥99%
Supelco
Ethyl palmitate, analytical standard
Sigma-Aldrich
Indomethacin, 98.5-100.5% (in accordance with EP)
Sigma-Aldrich
Indomethacin, meets USP testing specifications
Indomethacin, European Pharmacopoeia (EP) Reference Standard
Supelco
Indomethacin, Pharmaceutical Secondary Standard; Certified Reference Material
USP
Indomethacin, United States Pharmacopeia (USP) Reference Standard