MAB3408
Anti-Tubulin Antibody, beta, clone KMX-1
clone KMX-1, Chemicon®, from mouse
Synonym(s):
Tubulin beta-III, tubulin, beta 3, tubulin, beta 4
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
KMX-1, monoclonal
species reactivity
human, rat, mouse
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2b
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TUBB3(10381)
General description
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low and therefore beta Tubulin should not be used as loading control for these tissues.
Specificity
This antibody reacts specifically with β-tubulin and shows a strong cross-reactivity to different species. This antibody allows staining of microtubuli using indirect immunofluorescence showing a low background only.
Immunogen
Tubulin purified from polycephalum myxamoebae
Application
Immunocytochemistry:
A 1-2 μg/mL concentration of a previous lot was used in immunocytochemistry.
Western blot:
0.05-0.5 μg/mL
Optimal working dilutions must be determined by end user.
A 1-2 μg/mL concentration of a previous lot was used in immunocytochemistry.
Western blot:
0.05-0.5 μg/mL
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
This Anti-Tubulin Antibody, beta, clone KMX-1 is validated for use in IC, WB for the detection of Tubulin.
Quality
Routinely evaluated by Western Blot on A431 lysates.
Western blot:
1:500 dilution of this lot detected TUBULIN (BETA) on 10 μg of A431 lysates.
Western blot:
1:500 dilution of this lot detected TUBULIN (BETA) on 10 μg of A431 lysates.
Target description
50 kDa
Linkage
Replaces: 04-1049
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal IgG2b liquid in buffer containing 0.02M phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Storage and Stability
Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
Analysis Note
Control
HeLa Cell lysate
A431 Cell lysate
MCF7 Cell lysate
HeLa Cell lysate
A431 Cell lysate
MCF7 Cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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