CRISPR Essentials: MISSION® CRISPR gRNAs, Cas9 and Related Products
Compare and contrast the features of CRISPR ribonucleoprotein (RNP) products: guide RNA (gRNA) and Cas9 products for in vitro and in vivo CRISPR experiments including knockout/knock-in, activation, and inhibition. Select the best formats for your specific applications from synthetic gRNA, plasmid DNA, lentiviral particles, and proteins.
Section Overview
CRISPR RNP
Single-guide Synthetic RNA
Synthetic CRISPR gRNAs with guaranteed activity
Product | Description | Specifications Available | Product Availability |
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PURedit™ Synthetic gRNA | Choose from our collection of predesigned guides optimized for knockout in human and mouse genomes or design and order custom sequences. |
| Ships in 7-10 business days |
Standard Synthetic gRNA | Choose from our collection of predesigned guides optimized for knockout in human and mouse genomes or design and order custom sequences. |
| Ships in 5-7 business days |
Two-part Synthetic gRNA | |||
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Product | Description | Specifications Available | Product Availability |
Synthetic crRNA | Choose from our collection of predesigned guides optimized for knockout in human and mouse genomes or design and order custom sequences. |
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TRACRRNA05N | Synthetic SpCas9 tracrRNA | 5 nmol | Ready to ship |
TRACRRNAMOD | Synthetic SpCas9 modified tracrRNA Pair with modified crRNA | 5 nmol | Ready to ship |
CRISPR two-part system requires both crRNA and tracrRNA to complex with Cas9.
Cas9 Proteins | ||
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Product No. | Product | Description/Application |
PECAS9 | PURedit™ Cas9 | PURedit™ SpCas9 with enhanced quality and high efficiency. Engineered for high on-target activity without compromising in specificity. Ideal for preclinical and translational applications including cell-based therapies. |
CAS9PL | Cas9 Plus | High efficiency SpCas9 variant that gives both high on-target activity and specificity. Ideal for applications requiring high accuracy. |
CAS9GFPPRO | Wild-type SpCas9-EGFP Fusion | Original Wild-Type Cas9 fused to enhanced green fluorescent protein. Ideal for flow cytometry applications and visualization of transfected RNP complexes. Highly efficient |
ECAS9GFPPR | Enhanced specificity SpCas9-EGFP Fusion | Cas9 variant with Improved specificity fused to enhanced green fluorescent protein. Ideal for flow cytometry applications, fluorescence microscopy and for experiments that require high accuracy |
CAS9PROT | Wild-type SpCas9 | Original , wild-type Cas9; simple and economical; efficient in a broad range of gene editing applications |
ESPCAS9PRO | Enhanced specificity SpCas9 | Cas9 variant with improved specificity ideal for experiments that require high accuracy |
CAS9D10APR | Cas9D10A Nickase | A two-gRNA system with improved specificity and decreased off-target potential for specialized genome-editing applications |
DCAS9PROT | dCas9-3XFLAG™ Biotin | Catalytically inactive SpCas9 protein containing a 3XFLAGTM tag and Biotin; ideal for DNA detection and isolation and is a component of the proxy-CRISPR method |
FNCAS9PROT | FnCas9 Protein | Ideal for synthetic biology or cell-free applications |
mRNA, PLASMIDS, AND LENTIVIRUS
Sanger QuickPick™ Knockout Pre-cloned Guides | ||
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Product | Description | Product Availability |
Sanger QuickPick™ Knockout Pre-cloned Guides | Choose ready-to-use guides from the Sanger Whole Genome Knockout Library for human and mouse, pre-cloned into the Sanger vector backbone. | Ready to ship |
Cas9 Products | ||
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Product No. | Description | Format |
CAS9P | SpCas9 plasmid | Plasmid DNA |
CAS9GFPP | SpCas9 GFP Plasmid | Plasmid DNA |
CAS9RFPP | SpCas9 RFP Plasmid | Plasmid DNA |
CAS9GEMP | SpCas9 GFP Geminin Plasmid | Plasmid DNA |
CAS9D10AP | SpCas9-D10A Nickase Plasmid | Plasmid DNA |
CAS9D10AGFPP | SpCas9-D10A Nickase GFP Plasmid | Plasmid DNA |
CAS9D10ARFPP | SpCas9-D10A Nickase RFP Plasmid | Plasmid DNA |
DCAS9P | SpdCas9 Plasmid | Plasmid DNA |
DCAS9P300 | SpdCas9-p300 Activator Plasmid | Plasmid DNA |
DCAS9P300RFP | SpdCas9-p300 RFP Activator Expression Plasmid | Plasmid DNA |
FNCAS9P | FnCas9 Plasmid | Plasmid DNA |
CAS9MRNA | SpCas9 mRNA | mRNA |
CAS9D10AMRNA | SpCas9-D10A Nickase mRNA | mRNA |
CAS9BST | SpCas9 Blasticidin Lenti Plasmid | Plasmid DNA |
CAS9HYGROVP | SpCas9 Hygromycin Lenti Plasmid | Plasmid DNA |
CAS9NEO | SpCas9 Neomycin Lenti Plasmid | Plasmid DNA |
CAS9ZEOVP | SpCas9 Zeo Lenti Plasmid | Plasmid DNA |
CAS9GFPVP | SpCas9 GFP Lenti Plasmid | Plasmid DNA |
CAS9RFPVP | SpCas9 RFP Lenti Plasmid | Plasmid DNA |
ESPCAS9BST | High Fidelity Sp Cas9 Blasticidin Lenti Plasmid | Plasmid DNA |
LVCAS9BST | SpCas9 Blasticidin Lenti Particles | Lentivirus |
LVCAS9NEO | SpCas9 Neomycin Lenti Particles | Lentivirus |
CAS9BAC1P | SpCas9 Lambda Red Homologous Recombination Plasmid for E.coli | Plasmid DNA |
Product No. | Description | Compatible gRNA vector | System | Format | Selection Method | Comment |
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SAMHELPERV | SAM CRISPRa Helper Construct Kit Lentiviral Transduction Particles | LV06 & LV07 | Lentiviral | Lentivirus | Blasticidin & Hygromycin | Order compatible gRNA separately Kit contains 2 components:
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SAMHELPERP | SAM CRISPRa Helper Construct Kit Plasmid DNA | LV06 & LV07 | Lentiviral | Plasmid DNA | Blasticidin & Hygromycin | Order compatible gRNA separately Kit contains 2 components:
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SAMVP64BSTV | dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 Lentiviral Transduction Particles | LV06 & LV07 | Lentiviral | Lentivirus | Blasticidin | Order compatible gRNA separately Requires MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2 |
SAMVP64BST | dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 Plasmid DNA | LV06 & LV07 | Lentiviral | Plasmid DNA | Blasticidin | Order compatible gRNA separately Requires MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2 |
SAMMS2HYGV | MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2 Lentiviral Transduction Particles | LV06 & LV07 | Lentiviral | Lentivirus | Hygromycin | Order compatible gRNA separately Requires dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 |
SAMMS2HYG | MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2 Plasmid DNA | LV06 & LV07 | Lentiviral | Plasmid DNA | Hygromycin | Order compatible gRNA separately Requires dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 |
CRISPRICON | CRISPRi Control & Effector Kit | LV17 | Lentiviral | Lentivirus | Puromycin & BFP |
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CRISPRI10X | 10X CRISPRi Feature Barcode Optimization Kit | LV13 LV14 LV15 LV16 | Lentiviral | Lentivirus | Puromycin & BFP |
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CRISPR Controls
Negative Controls | ||||||
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Product No. | Description | Vector | Control Type | System | Format | gRNA Type |
CRISPR06 | CRISPR Universal Non-target Negative Control 1, Cas9+gRNA Plasmid | p01 | Neg. | Mammalian | Plasmid DNA | Cas9+gRNA |
CRISPR07 | CRISPR Universal Non-target Negative Control 2, Cas9+gRNA Plasmid | p01 | Neg. | Mammalian | Plasmid DNA | Cas9+gRNA |
CRISPR08 | CRISPR Universal Non-target Negative Control 3, Cas9+gRNA Plasmid | p01 | Neg. | Mammalian | Plasmid DNA | Cas9+gRNA |
CRISPR17 | CRISPR Activator Human Oct4 Positive Control Plasmid, gRNA Only Expression Plasmid | p03 | Pos. | Mammalian | Plasmid DNA | gRNA only |
CRISPR12 | CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lenti Plasmid (vector) | LV01 | Neg. | Lentiviral | Plasmid DNA | Cas9+gRNA |
CRISPR18 | CRISPR-Lenti Universal Non-target Negative Control 1, gRNA Only Lenti Plasmid (Sanger vector) | LV04 | Neg. | Lentiviral | Plasmid DNA | gRNA only |
CRISPR19 | CRISPR-Lenti Universal Non-target Negative Control 2, gRNA Only Lenti Plasmid (Sanger vector) | LV04 | Neg. | Lentiviral | Plasmid DNA | gRNA only |
CRISPR20 | CRISPR-Lenti Universal Non-target Negative Control 3, gRNA Only Lenti Plasmid (Sanger vector) | LV04 | Neg. | Lentiviral | Plasmid DNA | gRNA only |
CRISPR12V | CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lentiviral Particles (vector) | LV01 | Neg. | Lentiviral | Lentivirus | Cas9+gRNA |
CRISPR12H | CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lentiviral Particles, High Titer (vector) | LV01 | Neg. | Lentiviral | Lentivirus | Cas9+gRNA |
CRISPR15H | Gecko2-Lenti Universal Non-Target Negative Control 1, gRNA Only High Titer Lentiviral Particles (Gecko2 vector) | LV02 | Neg. | Lentiviral | Lentivirus | gRNA only |
CRISPR18V | CRISPR-Lenti Universal Non-target Negative Control 1, gRNA Only Lentiviral Particles (Sanger vector) | LV04 | Neg. | Lentiviral | Lentivirus | gRNA only |
CRISPR19V | CRISPR-Lenti Universal Non-target Negative Control 2, gRNA Only Lentiviral Particles (Sanger vector) | LV04 | Neg. | Lentiviral | Lentivirus | gRNA only |
CRISPR20V | CRISPR-Lenti Universal Non-target Negative Control 3, gRNA Only Lentiviral Particles (Sanger vector) | LV04 | Neg. | Lentiviral | Lentivirus | gRNA only |
CRISPR01 | CRISPR Human EMX1 Positive Control Plasmid, gRNA Only Expression Plasmid, includes Cas9 Expression Plasmid (vector) | p03 | Pos. | Mammalian | Plasmid DNA | gRNA only |
CRISPR02 | CRISPR Nickase Human EMX1 Positive Control Plasmids (gRNA Only Expression Plasmids, Includes Cas9-D10A Plasmid) | p03 | Pos. | Mammalian | Plasmid DNA | gRNA only |
CRISPR11 | CRISPR-Lenti Human EMX1 Positive Control, Cas9+gRNA Lenti Plasmid (vector) | LV01 | Pos. | Lentiviral | Plasmid DNA | Cas9+gRNA |
CRISPR13 | CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lenti Plasmid (vector) | LV01 | Pos. | Lentiviral | Plasmid DNA | Cas9+gRNA |
CRISPR21 | CRISPR-Lenti Human HPRT1 Positive Control, gRNA Only Lenti Plasmid (Sanger vector) | LV04 | Pos. | Lentiviral | Plasmid DNA | gRNA only |
CRISPR11V | CRISPR-Lenti Human EMX1 Positive Control, Cas9+gRNA Lentiviral Particles (vector) | LV01 | Pos. | Lentiviral | Lentivirus | Cas9+gRNA |
CRISPR13V | CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lentiviral Particles (vector) | LV01 | Pos. | Lentiviral | Lentivirus | Cas9+gRNA |
CRISPR13H | CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lentiviral Particles, High Titer (vector) | LV01 | Pos. | Lentiviral | Lentivirus | Cas9+gRNA |
CRISPR16H | Gecko2-Lenti Human HPRT1 Positive Control, gRNA Only High Titer Lentiviral Particles (Gecko2 vector) | LV02 | Pos. | Lentiviral | Lentivirus | gRNA only |
CRISPR21V | CRISPR-Lenti Human HPRT1 Positive Control, gRNA Only Lentiviral Particles (Sanger vector) | LV04 | Pos. | Lentiviral | Lentivirus | gRNA only |
CRISPRPL01 | CRISPR GUS GAPDH Reporter Control for Monocots | p62 | Pos. | Plant | Plasmid DNA | Cas9+gRNA |
CRISPRPL02 | CRISPR GUS GAPDH Reporter Control for Dicots | p63 | Pos. | Plant | Plasmid DNA | Cas9+gRNA |
Positive Controls | ||||||
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Product No. | Description | Vector | Control Type | System | Format | gRNA Type |
CRISPR01 | CRISPR Human EMX1 Positive Control Plasmid, gRNA Only Expression Plasmid, includes Cas9 Expression Plasmid (vector) | p03 | Pos. | Mammalian | Plasmid DNA | gRNA only |
CRISPR02 | CRISPR Nickase Human EMX1 Positive Control Plasmids (gRNA Only Expression Plasmids, Includes Cas9-D10A Plasmid) | p03 | Pos. | Mammalian | Plasmid DNA | gRNA only |
CRISPR11 | CRISPR-Lenti Human EMX1 Positive Control, Cas9+gRNA Lenti Plasmid (vector) | LV01 | Pos. | Lentiviral | Plasmid DNA | Cas9+gRNA |
CRISPR13 | CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lenti Plasmid (vector) | LV01 | Pos. | Lentiviral | Plasmid DNA | Cas9+gRNA |
CRISPR21 | CRISPR-Lenti Human HPRT1 Positive Control, gRNA Only Lenti Plasmid (Sanger vector) | LV04 | Pos. | Lentiviral | Plasmid DNA | gRNA only |
CRISPR11V | CRISPR-Lenti Human EMX1 Positive Control, Cas9+gRNA Lentiviral Particles (vector) | LV01 | Pos. | Lentiviral | Lentivirus | Cas9+gRNA |
CRISPR13V | CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lentiviral Particles (vector) | LV01 | Pos. | Lentiviral | Lentivirus | Cas9+gRNA |
CRISPR13H | CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lentiviral Particles, High Titer (vector) | LV01 | Pos. | Lentiviral | Lentivirus | Cas9+gRNA |
CRISPR16H | Gecko2-Lenti Human HPRT1 Positive Control, gRNA Only High Titer Lentiviral Particles (Gecko2 vector) | LV02 | Pos. | Lentiviral | Lentivirus | gRNA only |
CRISPR21V | CRISPR-Lenti Human HPRT1 Positive Control, gRNA Only Lentiviral Particles (Sanger vector) | LV04 | Pos. | Lentiviral | Lentivirus | gRNA only |
CRISPRPL01 | CRISPR GUS GAPDH Reporter Control for Monocots | p62 | Pos. | Plant | Plasmid DNA | Cas9+gRNA |
CRISPRPL02 | CRISPR GUS GAPDH Reporter Control for Dicots | p63 | Pos. | Plant | Plasmid DNA | Cas9+gRNA |
NON-MAMMALIAN CRISPR SYSTEMS
Plant CRISPR
Explore ready-to-use cas9 and guide RNA (gRNA) expression plasmids for monocots and dicots
- Our plant CRISPR/Cas9 products are intended for Agrobacterium-mediated plant transformation or biolistic microparticle bombardment or protoplast transformation
- A codon optimized Cas9 protein and a gRNA are expressed from a single vector and provided as ready-to-use, transfection-grade DNA.
Bacterial CRISPR
Our E. coli CRISPR/Cas-mediated Lambda-Red (λ-Red) homologous recombination (HR) vector system facilitates gene editing through the homology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either ssDNA or dsDNA as an editing template.
Vectors
Mammalian System Vectors | ||||||
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Vector No. | Description | Order Clones | Cas9/gRNA Content | Format Availability | Other Features | |
Fluorophore | Cas9 type | |||||
p01 | U6-gRNA:CMV-Cas9-2A-tGFP | p01 clones | Cas9+gRNA | Plasmid DNA | GFP | S. pyogenes |
p02 | U6-gRNA:CMV-Cas9-2A-tRFP | p02 clones | Cas9+gRNA | Plasmid DNA | RFP | S. pyogenes |
p04 | U6-gRNA:CMV-eCas9-2a-tGFP | p04 clones | Cas9+gRNA | Plasmid DNA | GFP | enhanced specificity |
p03 | U6-gRNA | p03 clones | gRNA only | Plasmid DNA |
Lentiviral Vectors | |||||
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Vector No. | Description | Order Clones | Cas9/gRNA Content | Format Availability | Selection Method |
LV01 | U6-gRNA:ef1a-puro-2A-Cas9-2A-tGFP | LV01 clones | Cas9+gRNA | Plasmid DNA & Lentivirus | Fluorophore (GFP) & Puromycin |
LV05 | U6-gRNA:EF1a-Cas9+FLAG-2A-Puro | LV05 clones | Cas9+gRNA | Plasmid & Lentivirus | Puromycin |
LV06 | CRISPRa SAM U6-gRNA:EF1a-Puro | LV06 clones | CRISPR activator gRNA | Plasmid & Lentivirus | Puromycin |
LV07 | CRISPRa SAM U6-gRNA:EF1a-Zeo | LV07 clones | CRISPR activator gRNA | Plasmid & Lentivirus | Zeo |
LV04 | U6-gRNA:hPGK-puro-2A-tBFP | Sanger QP KO clones | gRNA only | Plasmid DNA & Lentivirus | Fluorophore (BFP) & Puromycin |
LV03 | U6-gRNA:ef1a-tGFP | LV03 clones | gRNA only | Plasmid DNA & Lentivirus | Fluorophore (GFP) |
LV02 | U6-gRNA:ef1a-puro | LV02 clones | gRNA only | Plasmid DNA & Lentivirus | Puromycin |
Plant Vectors | |||||||
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Vector No. | Description | Order Clones | Cas9/gRNA Content | Format Availability | Species | Plasmid Type | Selection |
p63 | (dicot)U6-gRNA:CMV-Cas9-beta glucuronidase (Agrobacterium plasmid) | p63 clones | Cas9+ gRNA | Plasmid DNA | Dicot | Agro-bacterium | Beta Glucuron- idase |
p55 | (dicot)U6-gRNA:CMV-Cas9-bar (Agrobacterium plasmid) | p55 clones | Cas9+ gRNA | Plasmid DNA | Dicot | Agro-bacterium | Biala- phos |
p53 | (dicot)U6-gRNA:CMV-Cas9-hyg (Agrobacterium plasmid) | p53 clones | Cas9+ gRNA | Plasmid DNA | Dicot | Agro-bacterium | Hygro- mycin |
p54 | (dicot)U6-gRNA:CMV-Cas9-neo (Agrobacterium plasmid) | p54 clones | Cas9+ gRNA | Plasmid DNA | Dicot | Agro-bacterium | Neo- mycin |
p61 | (dicot)U6-gRNA:CMV-Cas9-bar (bioballistic plasmid) | p61 clones | Cas9+ gRNA | Plasmid DNA | Dicot | Bio-Ballistic | Biala- phos |
p59 | (dicot)U6-gRNA:CMV-Cas9-hyg (bioballistic plasmid) | p59 clones | Cas9+ gRNA | Plasmid DNA | Dicot | Bio-Ballistic | Hygro- mycin |
p60 | (dicot)U6-gRNA:CMV-Cas9-neo (bioballistic plasmid) | p60 clones | Cas9+ gRNA | Plasmid DNA | Dicot | Bio-Ballistic | Neo- mycin |
p62 | (monocot)U6-gRNA:CMV-Cas9-beta glucuronidase (Agrobacterium plasmid) | p62 clones | Cas9+ gRNA | Plasmid DNA | Monocot | Agro-bacterium | Beta Glucuron- idase |
p52 | (monocot)U6-gRNA:CMV-Cas9-bar (Agrobacterium plasmid) | p52 clones | Cas9+ gRNA | Plasmid DNA | Monocot | Agro-bacterium | Biala- phos |
p50 | (monocot) U6-gRNA:CMV-Cas9-hyg (Agrobacterium plasmid) | p50 clones | Cas9+ gRNA | Plasmid DNA | Monocot | Agro-bacterium | Hygro- mycin |
p51 | (monocot)U6-gRNA:CMV-Cas9-neo (Agrobacterium plasmid) | p51 clones | Cas9+ gRNA | Plasmid DNA | Monocot | Agro-bacterium | Neo- mycin |
p58 | (monocot)U6-gRNA:CMV-Cas9-bar (bioballistic plasmid) | p58 clones | Cas9+ gRNA | Plasmid DNA | Monocot | Bio-Ballistic | Biala- phos |
p56 | (monocot)U6-gRNA:CMV-Cas9-hyg (bioballistic plasmid) | p56 clones | Cas9+ gRNA | Plasmid DNA | Monocot | Bio-Ballistic | Hygro- mycin |
p57 | (monocot)U6-gRNA:CMV-Cas9-neo (bioballistic plasmid) | p57 clones | Cas9+ gRNA | Plasmid DNA | Monocot | Bio-Ballistic | Neo- mycin |
Bacterial Vectors | |||||
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Vector No. | Description | Order Clones | Cas9/gRNA Content | Format Availability | Selection |
p10 | (bacteria) J23119-gRNA-amp | p10 clones | gRNA only | Plasmid DNA | Ampicillin |
Vector Maps and Sequences
Click on the images below for a larger view.
If you need any further information, please contact us here and a customer service representative will get back to you.
Product Guarantee
We are so confident in the performance of our synthetic gRNA products, that we fully guarantee the quality and performance of any gRNA we produce, including custom sequences. If your crRNA or sgRNA do not yield detectable cleavage at the intended target site, we will provide you a one-time replacement, free of charge.
To qualify for this guarantee, please send an image or sequencing data from a single experiment demonstrating detectable cleavage using one of our positive controls, side-by-side with the negative results from your synthetic gRNA. To receive your replacement, simply email oligotechserv@milliporesigma.com and include sample data from a representative experiment (T7E1, TIDE, or NGS).
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