Pyrosequencing to detect mutations in FKS1 that confer reduced echinocandin susceptibility in Candida albicans.

Pyrosequencing to detect mutations in FKS1 that confer reduced echinocandin susceptibility in Candida albicans.

Antimicrobial agents and chemotherapy (2008-09-17)

Nathan P Wiederhold, Jodi L Grabinski, Guillermo Garcia-Effron, David S Perlin, Samuel A Lee

RESUMEN

Pyrosequencing was compared to Sanger dideoxy sequencing to detect mutations in FKS1 responsible for reduced echinocandin susceptibility in Candida albicans. These methods were in complete agreement for 10 of 12 clinical isolates with elevated echinocandin MICs, supporting the potential feasibility of pyrosequencing to detect mutations within diploid fungi.

MATERIALES

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H4522

Sigma-Aldrich

Suero humano, from human male AB plasma, USA origin, sterile-filtered

H3667

Sigma-Aldrich

Suero humano, Heat Inactivated, from human male AB plasma, USA origin, sterile-filtered

H6914

Sigma-Aldrich

Suero humano, (from male AB clotted whole blood), USA origin, sterile-filtered

H5667

Sigma-Aldrich

Suero humano, Heat Inactivated (from male AB clotted whole blood), USA origin, sterile-filtered

P2918

Sigma-Aldrich

Suero humano, from platelet poor human plasma, sterile-filtered, (mycoplasma tested, virus tested)