SAB4200235

Anti-TGN46 antibody, Mouse monoclonal

clone TGN46-52, purified from hybridoma cell culture

• Sinónimos: Anti-Trans-Golgi network protein, 46-kD
• MDL number: MFCD09265378
• UNSPSC Code: 12352203
• NACRES: NA.41

Propiedades

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: TGN46-52, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 80-100 kDa
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): immunoprecipitation (IP): suitable; western blot: 1-2 μg/mL using whole extracts of HEK-293T cells over-expressing human TGN46
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TGN46(10618)

Descripción

General description

Monoclonal Anti-TGN46 (mouse IgG1 isotype) is derived from the hybridoma TGN46-52 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human TGN46 conjugated to KLH. Trans-Golgi network protein 46-kDa (TGN46), It is a heavily glycosylated protein, containing a signal peptide, a lumenal domain, a membrane-spanning domain and a cytoplasmic domain. The membrane spanning region and the cytoplasmic tail contain the retention and retrieval signals for localization in the TGN.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-TGN46 antibody produced in mouse has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Trans-Golgi network protein, 46-kDa (TGN46) is involved in regulating membrane traffic to and from the TGN. It cycles constitutively between the TGN and the plasma membrane, returning via endosomes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable