M8195

Monoclonal Anti-phospho-MDMX (pTyr⁹⁹) antibody produced in mouse

~2 mg/mL, clone PH-MDMX-169, purified immunoglobulin, buffered aqueous solution

• Sinónimos: Anti-HDM4, Anti-HDMX, Anti-MDM4, Anti-MGC132766, Anti-MRP1
• UNSPSC Code: 12352203
• NACRES: NA.41

Propiedades

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: PH-MDMX-169, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~80 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• concentration: ~2 mg/mL
• technique(s): indirect ELISA: suitable; indirect immunofluorescence: suitable; western blot: 1-2 μg/mL using total cell extract of HEK-293T cells co-transfected with human MDMX and a specific kinase
• isotype: IgG2b
• UniProt accession no.: O15151
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pTyr99)
• Gene Information: human ... MDM4(4194)

Descripción

General description

MDMX is a nuclear protein and the gene encoding it is localized on human chromosome 1q32.1.

Monoclonal Anti-phospho-MDMX (pTyr99) (mouse IgG2b isotype) is derived from the hybridoma PH-MDMX-169 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Mouse double minute 4 (MDM4) or MDMX comprises p53 binding domain, central acidic region, zinc and interesting new gene (RING) finger domain and is a phosphorylated protein. MDM4 gene is mapped to human chromosome 1q32.1.

Specificity

Monoclonal Anti-phospho-MDMX (pTyr99) recognizes human phosphorylated MDMX (pTyr99) (~ 80 kDa). The antibody does not recognize the non-phosphorylated MDMX, which is produced when MDMX is expressed with a kinase mutant.

Immunogen

synthetic phosphopeptide corresponding to amino acids 93-106 (pTyr⁹⁹) of human MDMX.

Application

Monoclonal Anti-phospho-MDMX (pTyr99) antibody produced in mouse may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunofluorescence
• immunoblotting

Biochem/physiol Actions

MDMX associates with tumor suppressor p53 and inhibits its activity. It is upregulated in many tumors.

Studies began to unravel the regulation of murine double minute X (MDMX) in response to stress conditions, involving post-translational modifications and protein-protein interactions.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discard if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)