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Key Documents

GE17-0906-01

NHS Act Sepharose™ 4 Fast Flow

Cytiva 17-0906-01, pack of 25 mL

Sinónimos:

NHS-Activated Sepharose FF, pre-activated agarose matrix

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

N-hydroxysuccinimide activated groups

packaging

pack of 25 mL

manufacturer/tradename

Cytiva 17-0906-01

storage condition

(100% Isopropanol)

matrix

4% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

3-13(Ligand dependent)

ligand dependent

3-13

working range

3-12(3 (ligand dependent)-12 (ligand dependent))

suitability

suitable for bioprocess medium

storage temp.

2-8°C

General description

NHS-activated Sepharose™ 4 Fast Flow is a pre-activated agarose matrix that increases the choice of coupling chemistries available. Suitable for coupling amino-containing smaller proteins and peptides.

NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups.

This resin provides a spacer arm to the coupled ligand and is therefore particularly suitable for immobilising smaller protein and peptide ligands.

NHS-activated Sepharose™ 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, NHS-activated Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • NHS pre-activated medium for coupling of small amino-containing proteins and peptides in process-scale applications
  • High level of pre-activation gives a high degree of substitution of the selected ligand
  • NHS coupling method results in chemically stable ligand attachment
  • BioProcess medium supported for industrial applications

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Exclamation mark
GHS07

signalword

Danger

Storage Class

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Column Packing and Preparation for Affinity Chromatography of Antibodies

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocolos

Coupling Protocol for Primary Amine of a Ligand

This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.

Column Packing and Preparation for Affinity Chromatography with Specific Groups of Biomolecules

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

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