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Key Documents

GE17-5113-01

Estreptavidina Sepharose™ Alto rendimiento

Cytiva 17-5113-01, pack of 5 mL

Sinónimos:

medio de cromatografía de afinidad, resina de afinidad de alto rendimiento, purificación de proteínas biotiniladas

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

packaging

pack of 5 mL

manufacturer/tradename

Cytiva 17-5113-01

storage condition

(20% Ethanol)

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning in place

2-10.5

working range

4-9

capacity

binding capacity(>300 nmol biotin/ml medium 6 mg biotinylated BSA/ml medium)

storage temp.

2-8°C

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General description

Estreptavidina Sepharose™ High Performance es un medio para cromatografía de afinidad diseñado para la purificación rápida y fiable de alta resolución de biomoléculas biotiniladas.

La estreptavidina purificada aislada de Streptomyces avidinii se inmoviliza en Sepharose™ High Performance. La matriz base es una agarosa granulada rígida muy reticulada y con gran estabilidad química. La estreptavidina inmovilizada une la biotina y sustancias biotiniladas mediante cromatografía de afinidad.

Features and Benefits

  • Estreptavidina inmovilizada en Sephose™ High Performance para la purificación de compuestos biotinilados
  • Extremadamente útil para explotar las fuertes interacciones de la biotina y la estreptavidina o las interacciones algo más débiles de la 2-iminobiotina y la estreptavidina.
  • Tamaño del gránulo de 34 μm para una mejor resolución

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

Para ver el certificado de análisis de este producto, visite www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Jérôme Zervudacki et al.
The EMBO journal, 37(14) (2018-06-07)
Mobilization of transposable elements (TEs) in plants has been recognized as a driving force of evolution and adaptation, in particular by providing genes with regulatory modules that impact their transcription. In this study, we employed an ATCOPIA93 long-terminal repeat (LTR)
Adar Hacohen et al.
Micromachines, 11(5) (2020-05-21)
The ability to manipulate and selectively position cells into patterns or distinct microenvironments is an important component of many single cell experimental methods and biological engineering applications. Although a variety of particles and cell patterning methods have been demonstrated, most
Song Zhu et al.
Theranostics, 11(7), 3359-3375 (2021-02-05)
Background: A metabolic "switch" from oxidative phosphorylation to glycolysis provides tumor cells with energy and biosynthetic substrates, thereby promoting tumorigenesis and malignant progression. However, the mechanisms controlling this metabolic switch in tumors is not entirely clear. Methods: Clinical specimens were
Hana Antonicka et al.
Cell metabolism, 32(3), 479-497 (2020-09-03)
We used BioID, a proximity-dependent biotinylation assay with 100 mitochondrial baits from all mitochondrial sub-compartments, to create a high-resolution human mitochondrial proximity interaction network. We identified 1,465 proteins, producing 15,626 unique high-confidence proximity interactions. Of these, 528 proteins were previously
Sarah van Veen et al.
Nature, 578(7795), 419-424 (2020-01-31)
ATP13A2 (PARK9) is a late endolysosomal transporter that is genetically implicated in a spectrum of neurodegenerative disorders, including Kufor-Rakeb syndrome-a parkinsonism with dementia1-and early-onset Parkinson's disease2. ATP13A2 offers protection against genetic and environmental risk factors of Parkinson's disease, whereas loss

Artículos

Converting from Flow Velocity to Volumetric Flow Rates

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocolos

Performing a Separation of Biotin and Biotinylated Substances with HiTrap Streptavidin HP and Streptavidin Sepharose High Performance

How to separate biotin and biotinylated substances by affinity chromatography using HiTrap Streptavidin HP and Streptavidin Sepharose High Performance products from Cytiva.

Purification or Removal of Biotin and Biotinylated Substances

This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from Cytiva.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

Ver todo

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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