COS-7 Cell Line

87021302, African green monkey kidney, Fibroblast

• UNSPSC Code: 41106514

Propiedades

• product name: COS-7 Cell Line, from African green monkey kidney, 87021302, SV40 transformed, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• biological source: African green monkey kidney
• growth mode: Adherent
• karyotype: Not specified
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Monkey African green kidney, SV40 transformed

Cell Line Description

Derived from CV-1 simian cells transformed by an origin-defective mutant of SV40. The cells support the growth of recombinant SV40 viruses. The cells should be handled at laboratory containment level 2.

Application

COS-7 Cell Line has been used to:

• test the functional role of ankyrin B in ouabain/Na,K-ATPase/1,4,5-trisphosphate receptor (IP3R) signaling
• study the expression of green fluorescent protein-tagged constructs of neuropathy target esterase (NTE) and mutant proteins lacking the transmembrane, catalytic and regulatory domains
• study the distribution of peroxisomes

Virus replication studies: SV40

Culture Medium

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)..

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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