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B8894

Sigma-Aldrich

Albumin, Acetylated from bovine serum

protease free, for molecular biology

Sinónimos:

Ac-BSA, Acetyl BSA, Acetylated BSA

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About This Item

Número de CAS:
9048-46-8
EC Number:
232-936-2
MDL number:
MFCD01114300
UNSPSC Code:
12352202
NACRES:
NA.27

biological source

bovine

grade

for molecular biology

product line

BioReagent

form

solution

purified by

heat shock fractionation

contains

does not contain stabilizer or preservative

origin

USA origin

concentration

20 mg/mL in H2O

technique(s)

FISH: suitable

impurities

HIV I and HIVII, HCV and HBsAg, tested negative

UniProt accession no.

P02769

foreign activity

DNase free
DNase, RNase, NICKase and protease, none detected
NICKase free
Protease free
RNase free

storage temp.

−20°C

Gene Information

bovine ... ALB(280717)

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General description

Serum albumin is the most extensive plasma protein in the circulatory system. It is produced within the liver and exported as a non-glycosylated protein to the blood plasma. The molecular weight of native serum albumin is around 66 kDa. Native serum albumin is a negatively charged protein.

Acetylation of bovine serum albumin particularly modifies lysine (Lys) residues with elevated frequency, with lesser degrees of modification of serine (Ser) and threonine (Thr) residues. MALDI-TOF mass spectrometry analysis of acetylated BSA has indicated an increased MW of about 3000, compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. This acetylation process also derivatizes similar amino acid residues in any trace contaminating nucleases in the BSA, to inactivate that nuclease activity. This makes acetylated BSA useful in molecular biology applications where trace nuclease activity must be minimized.

Application

Select the right albumin for your application with the Albumin Selection Guide.
Acetylated BSA has been used as a component of the reaction mixture for adapter ligation. In addition, this B8894 acetylated BSA product has been used in other applications, such as:
  • In cDNA library preparation (Hafner, M. et al., RNA, 17(9), 1697-1712 (2011))
  • As a blocking agent with streptavidin beads used for capture of biotinylated RNA (Winz, M.-L. et al., Nat. Protocol., 12(1), 122-149 (2017))
Acetylated to inactivate nucleases commonly found in BSA. Acetylated BSA should not be used as a protein standard since acetylation of tyrosine residues prevents color development in the Lowry assay and other similar protein determinations.

Biochem/physiol Actions

Native serum albumin plays a major role in maintaining the pH and osmotic pressure of the blood. It is also involved in transporting a diverse range of endogenous and exogenous metabolites, such as amino acids, fatty acids, drugs, steroids and metal ions. Native serum albumin can have a role in modulating the levels of Ca2+ and Mg2+ metals in blood.

Features and Benefits

This acetylated BSA product has been tested for the following properties:
  • DNase-free / Exonuclease-free
  • Nickase-free / Endonuclease-free
  • Protease-free
  • RNase-free

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Nature protocols, 10(5), 645-659 (2015-04-04)
The heterogeneity of DNA methylation within a population of cells necessitates DNA methylome profiling at single-cell resolution. Recently, we developed a single-cell reduced-representation bisulfite sequencing (scRRBS) technique in which we modified the original RRBS method by integrating all the experimental
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STAR protocols, 2(4), 100901-100901 (2021-11-25)
Eukaryotic RNAs can be modified with a non-canonical 5' nicotinamide adenine dinucleotide (NAD+) cap. NAD-seq identifies transcriptome-wide NAD+ capped RNAs. NAD-seq takes advantage of click chemistry to allow the capture of NAD+ capped RNAs. Unlike other approaches, NAD-seq does not
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Aberrant induction of type I IFN is a hallmark of the inherited encephalopathy Aicardi-Goutières syndrome (AGS), but the mechanisms triggering disease in the human central nervous system (CNS) remain elusive. Here, we generated human models of AGS using genetically modified
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Methods (San Diego, Calif.), 44(1), 3-12 (2007-12-26)
Distinct classes of small RNAs, 20-32 nucleotides long, play important regulatory roles for diverse cellular processes. It is therefore important to identify and quantify small RNAs as a function of development, tissue and cell type, in normal and disease states.
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