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Merck
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Key Documents

A9231

Sigma-Aldrich

Acridine Orange solution

2% in H2O

Sinónimos:

3,6-Acridinediamine, N,N,N′,N′-tetramethyl-, monohydrochloride, 3,6-Bis(dimethylamino)acridine hydrochloride

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About This Item

Fórmula empírica (notación de Hill):
C17H19N3 · HCl
Número de CAS:
65-61-2
Peso molecular:
301.81
MDL number:
MFCD00005029
UNSPSC Code:
12171500
PubChem Substance ID:
329771043
NACRES:
NA.47

form

liquid

Quality Level

100

shelf life

18 mo.

concentration

2% in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

InChI

1S/C17H19N3/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14/h5-11H,1-4H3

InChI key

DPKHZNPWBDQZCN-UHFFFAOYSA-N

Categorías relacionadas

General description

Acridine Orange (C.l. 46005) is an acridine dye with a ring structure and overall small planar conjugated system.

Application

Acridine orange is a DNA intercalating dye that is a recommended DNA stain for flow cytometry. It differentially stains double-stranded and single-stranded nucleic acids.

It also has the following applications-
  • staining of RNA and acid mucins
  • detecting apoptosis
  • staining blood smears to diagnose malaria
  • identifying and enumerating granulocytes in fish
  • staining eukaryotic cells to demonstrate low pH structures

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Matthew P Harris et al.
PLoS genetics, 4(10), e1000206-e1000206 (2008-10-04)
The genetic basis of the development and variation of adult form of vertebrates is not well understood. To address this problem, we performed a mutant screen to identify genes essential for the formation of adult skeletal structures of the zebrafish.
Donald P Evenson
Methods in molecular biology (Clifton, N.J.), 927, 147-164 (2012-09-21)
The SCSA(®) is the pioneering assay for the detection of damaged sperm DNA and altered proteins in sperm nuclei via flow cytometry of acridine orange (AO) stained sperm. The SCSA(®) is considered to be the most precise and repeatable test
Q L Chen et al.
Talanta, 97, 48-54 (2012-07-31)
We have utilized various valving scheme to leverage purely rotation-regulated flow control to enable comprehensive cell-based bioassays (CBBs) on centrifuge-based lab-on-a-disc (LOAD). A LOAD has been developed to examine allergic degranulation from live basophils for allergens screening for the first
Marko Gerić et al.
Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering, 47(11), 1521-1527 (2012-06-19)
The present study aimed to characterize chemical properties of clay bricks containing 20 % of sewage sludge. After detection of potentially hazardous metals, we simulated precipitation exposure of such material to determine the amount of heavy metals that could leach
Hong-xiang Wang et al.
Zhonghua yi xue za zhi, 92(26), 1828-1831 (2012-09-05)
To ascertain the epididymal sperm nuclear maturity in obstructive azoospermia (OA) patients. A total of 81 infertile males from andriatry clinic of Renji hospital were selected, including 32 OA patients (OA group) and 49 asthenospermia patients (asthenospermia group). Another 32
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