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Merck

DIUTP-RO

Roche

Digoxigenina-11-dUTP estable a los álcalis

=85% (HPLC), solution, pkg of 25 μL (11573152910 [1 mM]), pkg of 125 μL (11573179910 [1 mM]]])

Sinónimos:

Digoxigenin-11-dUTP, alkali-labile, digoxigenin

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About This Item

UNSPSC Code:
41105500

Quality Level

assay

85% (HPLC)

form

solution

packaging

pkg of 125 μL (11573179910 [1 mM]]])
pkg of 25 μL (11573152910 [1 mM])

manufacturer/tradename

Roche

storage temp.

−20°C

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General description

Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP), alkali-labile is provided as in 1 mM tetralithium salt, solution. In which DIG is bound to dUTP via an alkali-sensitive ester linkage.

Application

Digoxigenin-11-dUTP, alkali-labile can be used for non-radioactive DNA labeling e.g. random primed or nick translation. It can also be used for labeling probes which are preferred in hybridization experiments where stripping and reprobing of the membrane is intended.

Biochem/physiol Actions

Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) replaces deoxythymidine triphophate (dTTP) in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% DIG-11-dUTP and 65% dTTP. It is ideal as a substrate for DNA polymerase, Taq DNA polymerase, terminal transferase and reverse transcriptase.

Other Notes

For life science research only. Not for use in diagnostic procedures.
When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will be removed. It is therefore ideal for use in DIG-labeled probes that can be removed by alkali denaturation (e.g ., in stripping and reprobing of blots). However, if you intend to make a DIG-labeled DNA that will survive alkaline treatment, do not use this alkali-labile preparation. Instead, use the alkali-stable form of DIG-11-dUTP.

also commonly purchased with this product

Referencia del producto
Descripción
Precios

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

does not flash

flash_point_c

does not flash


Certificados de análisis (COA)

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