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Key Documents

NS01L

Sigma-Aldrich

Normal Rabbit Serum

This Normal Rabbit Serum is validated for use in ELISA, Flow Cytometry, Immunoblotting, Immunofluorescence, Immunohistochemistry, Immunoprecipitation for the detection of Rabbit Serum.

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.41

biological source

rabbit

form

lyophilized

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

ambient

storage temp.

2-8°C

General description

Normal carrier sera from healthy, non-immunized rabbits.

Application

ELISA (see comments)

Flow Cytometry (see comments)

Immunoblotting (see comments)

Immunofluorescence (see comments)

Immunohistochemistry (see comments)

Immunoprecipitation (see comments)

Warning

Toxicity: Standard Handling (A)

Physical form

Serum diluted in 250 mM NaCl, 10 mM PBS, pH 7.6.

Reconstitution

Reconstitute the lypohilized diluted serum in 1 ml sterile distilled water with occasional gentle mixing for at least 2 h at 4°C. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.

Other Notes

Normal sera are provided to be used as blocking agents in immunohistochemical procedures or as negative controls for undiluted serum/rabbit polyclonal antibodies from the Calbiochem brand. For use as a negative control, perform experiments in parallel using primary antibody and normal serum at the same concentrations. A signal obtained with the normal serum indicates a nonspecific interaction; any additional signal with the primary antibody indicates a specific antibody-antigen interaction. This antibody has also been reported to work for ELISA, flow cytometry, immunoblotting, immunofluorescence, immunohistochemistry, and immunoprecipitation.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

hcodes

Hazard Classifications

Aquatic Chronic 3

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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