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PureProteome Protein G Magnetic Bead System

The PureProteome Protein G Magnetic Bead System is a powerful system that helps researchers purify proteins by maximizing recovery and eliminating variability

Sinónimos:

Protein G Magnetic Beads, PureProteome Magnetic Beads

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UNSPSC Code:
41105507
NACRES:
NA.56
eCl@ss:
32160405
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packaging

pkg of 2 × 1 mL

manufacturer/tradename

PureProteome

technique(s)

depletion: suitable (serum), immunoprecipitation (IP): suitable, protein purification: suitable

particle size

10 μm

capacity

2.5-3.5 μg/μL, suspension binding capacity (rabbit IgG)

shipped in

wet ice

General description

The PureProteome Protein G Magnetic Beads contain Protein G, a binding protein for antibodies, which is coated on the surface of the beads. These beads are designed to isolate immunoglobulins (IgG) from complex mixtures through antibody-affinity purification. These beads are used in conjunction with a magnetic stand to immobilize the beads. Following this step, the sample is washed to remove any impurities, and then the immobilized IgG can be eluted for further analysis or experimentation.

Application

PureProteome Protein G Magnetic Bead System is suitable for:
  • serum depletion
  • immunoprecipitation
  • protein purification    

PureProteome Protein G Magnetic Bead System is suitable:

  • for isolation of the immunoglobulin G (IgG) fraction from a small volume of plasma
  • for co-immunoprecipitation (Co-IP)

Features and Benefits

  • PureProteome Magnetic Beads are very well suited for fully automated purification process using the KingFisher Duo particle processor, it is not dependent on the cell lysate clarification step
  • The entire process is reproducible, and the results are comparable to that of a standard protocol using the PureProteome Magnetic Stand
  • PureProteome Magnetic Beads is compatible with any automated system for low, medium, or high-throughput sample preparation.

Legal Information

PureProteome is a trademark of Merck KGaA, Darmstadt, Germany

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Este artículo
LSKMAGALSKMAGHLSKMAGL10
manufacturer/tradename

PureProteome

manufacturer/tradename

PureProteome

manufacturer/tradename

PureProteome

manufacturer/tradename

PureProteome

particle size

10 μm

particle size

10 μm

particle size

10 μm

particle size

10 μm

packaging

pkg of 2 × 1 mL

packaging

pkg of 2 × 1 mL

packaging

pkg of 10 mL

packaging

pkg of 10 mL

capacity

2.5-3.5 μg/μL, suspension binding capacity (rabbit IgG)

capacity

1.5-2.5 μg/μL, suspension binding capacity (rabbit IgG)

capacity

1-5.5 mg/mL, bead suspension binding capacity (protein)

capacity

-

technique(s)

depletion: suitable (serum), protein purification: suitable, immunoprecipitation (IP): suitable

technique(s)

depletion: suitable (serum), immunoprecipitation (IP): suitable, protein purification: suitable

technique(s)

protein purification: suitable (histidine tagged recombinant protein)

technique(s)

depletion: suitable (serum), protein purification: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

ambient

shipped in

wet ice


Clase de almacenamiento

12 - Non Combustible Liquids

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

PureProteome™ Protein A and G Magnetic beads provide a rapid and reproducible means to purify immunoglobulins (IgG) using the KingFisher Duo particle processor.

Contenido relacionado

Immunoprecipitation (IP) is a powerful technique for proteomic screening, biomarker discovery, and signaling network elucidation. It is frequently used to enrich target proteins from complex samples such as cell lysates or extracts. Traditional IP protocols use Protein A, Protein G or a mixture of Protein A and G coupled to a solid support resin, such as agarose beads, to capture an antigen/antibody complex in solution. As the number of samples increase, the traditional, manual IP method can be time-consuming. Processing of multiple IP reactions in parallel can introduce complexity, variability and pipetting errors, which may affect reproducibility.

Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.

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SKUGTIN
LSKMAGG1004053252323188
LSKMAGG0204053252373053

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