182516-M

Arp2/3 Complex Inhibitor II, CK-869

The Arp2/3 Complex Inhibitor II, CK-869 controls the biological activity of Arp2/3. This small molecule/inhibitor is primarily used for Cell Structure applications.

• Sinónimos: Arp2/3 Complex Inhibitor II, CK-869, Actin Assembly Inhibitor XIV, 2-(3-Bromophenyl)-3-(2,4-dimethoxyphenyl)-1,3-thiazolidin-4-one, racemic, CK-0157869
• Fórmula empírica (notación de Hill): C₁₇H₁₆BrNO₃S
• Peso molecular: 394.28
• UNSPSC Code: 12352200

Propiedades

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: off-white
• solubility: DMSO: 50 mg/mL
• storage temp.: 2-8°C

Descripción

General description

A cell-permeable thiazolidinone compound that inhibits actin assembly mediated by actin-related protein Arp2/3 complex of human and bovine, but not yeast, species (IC₅₀ = 11 µM against bovine Arp2/3). Shown to inhibit the actin filament "comet tails" formation around intracellular Listeria in infected SKOV3 cells (IC₅₀ = 7 µM). Structrual studies indicate that CK-869 targets the hydrophobic core in subdomain 1 of Arp3. CK-666 (Cat. No. 182515) and CK-869 exhibit different modes of binding, resulting in their different yeast cross-reactivities. Supplied as a racemic mixture of the active 2S and the inactive 2R enantiomers. CK-312 (Cat. No. 182518) can serve as a negative control.

A cell-permeable thiazolidinone compound that inhibits actin assembly mediated by actin-related protein Arp2/3 complex of human and bovine, but not yeast, species (IC₅₀ = 11 µM against bovine Arp2/3). Shown to inhibit the actin filament "comet tails" formation around intracellular Listeria in infected SKOV3 cells (IC₅₀ = 7 µM). Structural studies indicate that CK-869 targets the hydrophobic core in subdomain 1 of Arp3. CK-666 and CK-869 exhibit different modes of binding, resulting in their different yeast cross-reactivities. Supplied as a racemic mixture of the active 2S and the inactive 2R enantiomers. CK-312 (Cat. No. 182518) can serve as a negative control.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Nolen, B.J., et al. 2009. Nature460, 1031.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable