06-1295
Anti-CDT1 Antibody
serum, from rabbit
Sinónimos:
DNA replication factor, Double parked homolog, Double parked, Drosophila, homolog of, chromatin licensing and DNA replication factor 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat, human
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CDT1(81620)
mouse ... Cdt1(67177)
rat ... Cdt1(292071)
General description
CDT1 (Chromatin Licensing and DNA replication Factor 1) is an essential component of the cell cycle machinery. It plays an important role in insuring that there is only one round of DNA replication per cell cycle. CDT1 is a known substrate of Cdk2 and Cdk4 (Liu, E. 2004). It is presented in G1 of the cell cycle and is degraded as cells enter S phase (Liu, E. 2004). In higher eukaryotes, CDT1 is known to interact with Geminin, a protein that is degraded during mitosis, that inhibits it. CDT1 is marked for degradation by two E3 ligases: SCF complex and the CUL4-DDB1 complex (Sansam, 2006).
Specificity
This antibody recognizes CDT1.
Immunogen
KLH-conjugated linear peptide corresponding to human CDT1.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Use Anti-CDT1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect CDT1 also known as DNA replication factor, Double parked homolog.
Western Blot Analysis: A 1:1,000 dilution from a previous lot detected CDT1 in C2C12, Hek293, HeLa, HepG2, Huvec, Jurkat, L6, mouse brain, NIH/3T3, PC3, and RAW264.7 cell lysates.
Quality
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected CDT1 in 10 µg of HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected CDT1 in 10 µg of HeLa cell lysate.
Target description
~60 kDa
Physical form
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Suzanne A Hartford et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(43), 17702-17707 (2011-10-12)
Effective DNA replication is critical to the health and reproductive success of organisms. The six MCM2-7 proteins, which form the replicative helicase, are essential for high-fidelity replication of the genome. Many eukaryotes have a divergent paralog, MCM9, that was reported
Richard O Adeyemi et al.
PLoS pathogens, 10(4), e1004055-e1004055 (2014-04-05)
Infection by the autonomous parvovirus minute virus of mice (MVM) induces a vigorous DNA damage response in host cells which it utilizes for its efficient replication. Although p53 remains activated, p21 protein levels remain low throughout the course of infection.
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