Skip to Content
Merck
  • Evaluation of the discriminative potential of a novel biomarker for estradiol treatments in bovine animals.

Evaluation of the discriminative potential of a novel biomarker for estradiol treatments in bovine animals.

Journal of agricultural and food chemistry (2014-12-09)
Patricia Regal, Marco H Blokland, Cristina A Fente, Saskia S Sterk, Alberto Cepeda, Leen A van Ginkel
ABSTRACT

The endogenous occurrence of natural hormones obstructs the application of classical targeted methods as confirmatory options. In the case of estradiol, the ultimate confirmation of its exogenous administration relies on gas chromatography coupled to combustion/isotope ratio mass spectrometry (GC-C/IRMS). A serum dipeptide composed of pyroglutamic acid and phenylalanine was identified as a potential biomarker of estradiol treatments in adult cows. To evaluate its potential to pinpoint suspicious samples, samples from prepubertal females under different estrogenic treatments have been analyzed. The results confirmed the up-regulation of the dipeptide in adult bovines. The 2-week-old females exhibited short-lasting responses only in a few animals. The 6-month-old female showed a delayed but clear increase on the biomarker level. The composition of the anabolic preparations, the dose, and/or the administration route are possible additional reasons for the reduced response in young animals. A comparison to previous results reported by various researchers is included.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
β-Estradiol 3-benzoate, ≥97%
Sigma-Aldrich
Methyl acetate, ≥98%, FG
Sigma-Aldrich
Methyl acetate, anhydrous, 99.5%
Supelco
Methyl acetate, analytical standard
Sigma-Aldrich
Methyl acetate, natural, 98%, FG
Sigma-Aldrich
Formic acid, ACS reagent, ≥88%
Sigma-Aldrich
Formic acid, reagent grade, ≥95%
Sigma-Aldrich
Formic acid, ≥95%, FCC, FG
Sigma-Aldrich
Formic acid solution, BioUltra, 1.0 M in H2O
Estradiol benzoate for system suitability, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Formic acid, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥98%
Estradiol benzoate, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Formic acid, ACS reagent, ≥96%
Sigma-Aldrich
Methyl acetate, ReagentPlus®, 99%
Sigma-Aldrich
Formic acid, puriss., meets analytical specifications of DAC, FCC, 98.0-100%
Sigma-Aldrich
Methyl acetate, suitable for HPLC, ≥99.8%