F3013

Ferredoxin from Spinacia oleracea (spinach)

lyophilized powder, Ferredoxin ≥15 wt. %

• CAS Number: 9040-09-9
• MDL number: MFCD00131049
• UNSPSC Code: 12352202
• NACRES: NA.61

Properties

• biological source: spinach (Spinacia oleracea)
• Quality Level: 200
• form: lyophilized powder
• composition: Ferredoxin, ≥15 wt. %
• solubility: water: 2 mg/mL, clear, faintly brown
• application(s): cell analysis
• storage temp.: −20°C
• Gene Information: spinach ... SOVF(137530)

Description

General description

Ferredoxin I (Fd I) from Spinacia oleracea comprises 97 amino acid and is an iron sulfur cluster (2Fe-2S). It is synthesized in the chloroplast. The structure of Fd I has conserved active site.

Application

Ferredoxin from Spinacia oleracea (spinach) has been used:

• as a substrate for spinach ferredoxin reductase,
• for reconstitution with chlorophyll-A for scanning electron microscope (SEM) dispersive X-ray analyzer (EDX) integrated analysis,
• as a reductant in adenylylsulfate (APS) reductase activity of P. aeruginosa

Biochem/physiol Actions

Ferredoxin is important in the electron transfer cascade required for this nitrogen assimilation. It has been used in studies of assimilation of inorganic nitrogen from nitrite or nitrate in the absence of ammonium.

Ferredoxins are iron-containing proteins that serve as electron-transfer catalysts in photosynthesis and nitrogen fixation.

Packaging

Package sizes are based on ferredoxin content.

Physical form

Contains Tris buffer

Preparation Note

Partially purified by the method of Tagawa and Arnon, Nature, 195, 537 (1962).

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315,H319,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves