FCABS400F

Milli-Mark^® Anti-FcεRI Antibody, γ subunit-FITC

Milli-Mark^®, from rabbit

• Synonym(s): Fc fragment of IgE high affinity I receptor for gamma polypeptide, Fc-epsilon RI-gamma, FceRI gamma, IgE Fc receptor subunit gamma, Immunoglobulin E receptor, high affinity,gamma chain
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: FITC conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, human, rat
• manufacturer/tradename: Milli-Mark^®
• technique(s): flow cytometry: suitable
• isotype: IgG
• NCBI accession no.: NP_004097
• UniProt accession no.: P30273
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... FCER1G(2207)

Description

General description

Type I allergic reactions are mediated through the IgE high affinity receptor (FceRI). When a multivalent antigen is presented, a redistribution of the monomeric IgE FceRI receptor complexes cause the degranulation of mast cells and basophils, resulting in the subsequent release of factors responsible for type I allergic reactions. The FceRI receptor is a tetrameric complex, consisting of an a-chain, b-chain and a dimeric g-chain. While the a-chain is primarily involved with the binding of IgE, the signal is transferred through the g subunit, which contains the immunoreceptor tyrosine activation motifs (ITAM) critical for initiating receptor mediated signal transduction through the Syk and Lyn tyrosine kinases. The FceRI receptor is able to engage and disengage the IgE, providing a versatile model for studying the function of kinase coupled receptors.

Specificity

Recognizes and is specific for FcεRI, γ-subunit.

Immunogen

Synthetic peptide corresponding to amino acids 80-86 of γ-subunit of Fc receptors.

Application

Milli-Mark Anti-FcεRI Antibody, γ subunit-FITC is an antibody against FcεRI for use in FC.

Research Category
Inflammation & Immunology

Research Sub Category
Inflammation & Autoimmune Mechanisms

Quality

Evaluated by flow cytometry using RBL cells.

Target description

10 kDa Calculated

Physical form

Protein A purified

Purified rabbit polyclonal IgG conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
RBL cells

Other Notes

Optimal working dilutions must be determined by end user for other instruments

Unconjugated antibody (Cat No. 06-727) has been shown to work on Human and Mouse.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable