M8440
Anti-MCH antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
MCH Antibody - Anti-MCH antibody produced in rabbit, Mch Antibody, Anti-Melanin-Concentrating Hormone
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About This Item
Recommended Products
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
human
technique(s)
indirect ELISA: 1:2,500
microarray: suitable
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PMCH(5367)
Related Categories
General description
Melanin-concentrating hormone (MCH) is a circulating 19-amino acid residue cyclic neuropeptide present in hypothalamus. In mammals, MCH is a, encoded by the precursor pro-melanin concentrating hormone (PMCH). It was first discovered in fish and subsequently in mammalian (rat). MCH perikarya is confined largely to the lateral hypothalamus area (LHA) and the adjacent zon incerta with extensive neuronal projections throughout the brain. MCH is detected in basal ganglia, neocortex, cerebellum, thymus, brown adipose tissue, duodenum, and testis. The structure of MCH is highly conserved among species.
Immunogen
synthetic peptide corresponding to human melanin-concentrating hormone conjugated to KLH. The MCH sequence is identical in many species including rat, mouse, and pig.
Application
Anti-MCH antibody may be used at a working dilution of 1:30000 for RIA. For immunohistochemistry of mouse hypothalamus sections, a working antibody dilution of 1:200 may be used. The antibody has been used for immunoblotting in cytoplasmic extracts of T cells at a concentration of 5.7 μg/ml. The antibody is suitable for protein microarray.
Anti-MCH antibody produced in rabbit has been used in radioimmunoassay and immunohistochemistry.
Biochem/physiol Actions
MCH mRNA is overexpressed and up-regulated during fasting in the hypothalamus of ob/ob mice as well as rats. Injection of MCH into the lateral ventricles in rats promote increased food consumption. In addition, knockout mice lacking MCH gene are lean and hypophagic and show an increase in metabolic rate. In contrast, overexpression of MCH in transgenic mice leads to obesity and insulin resistance.
Physical form
Solution in phosphate buffered saline containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Electrophysiological effects of MCH on neurons in the hypothalamus
Gao XB
Peptides, 30(11), 2025-2030 (2009)
N Kakava-Georgiadou et al.
Scientific reports, 9(1), 11146-11146 (2019-08-02)
Targeting specific neuronal cell types is a major challenge for unraveling their function and utilizing specific cells for gene therapy strategies. Viral vector tools are widely used to target specific cells or circuits for these purposes. Here, we use viral
Xuan Thang Pham et al.
PNAS nexus, 3(7), pgae275-pgae275 (2024-07-22)
Melanin-concentrating hormone-producing neurons (MCH neurons), found mainly in the lateral hypothalamus and surrounding areas, play essential roles in various brain functions, including sleep and wakefulness, reward, metabolism, learning, and memory. These neurons coexpress several neurotransmitters and act as glutamatergic neurons.
Mice lacking melanin-concentrating hormone are hypophagic and lean
Shimada M, et al.
Nature, 396(6712), 670-670 (1998)
Melanin-concentrating hormone overexpression in transgenic mice leads to obesity and insulin resistance
Ludwig DS, et al.
The Journal of Clinical Investigation, 107(3), 379-386 (2001)
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