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product name
293 N3S, 92052131
biological source
human kidney
growth mode
Suspension
karyotype
Not specified
morphology
Not specified
products
Adenovirus 5 early region (E1) antigens
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human embryo kidney
Cell Line Description
Adapted for growth in suspension by passaging 293 cells in nude mice. The cells express adenovirus 5 early region 1 (E1) antigens and are permissive for adenovirus 5 host range mutants defective in E1 functions.
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 12,14
D16S539: 9
D5S818: 9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19
CSF1PO: 11
D13S317: 12,14
D16S539: 9
D5S818: 9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19
Culture Medium
Joklik′s MEM + 2mM Glutamine + 10% Horse Serum.
Subculture Routine
Maintain cultures between 5 x 10,000 to 5 x 100,000 cells/ml in a Techne stirrer; 5% CO2; 37°C. Cell doubling time is 30 hours.
Other Notes
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Certificates of Analysis (COA)
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