11089153001
Roche
Streptavidin-POD conjugate
liquid, pkg of 1 mL
Synonym(s):
streptavidin
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About This Item
UNSPSC Code:
12352200
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General description
Streptavidin is covalently coupled to horseradish peroxidase. The conjugate is used for detection of biotin-labelled substances.
Application
Streptavidin-POD has been used for:
- ELISA
- In situ hybridization
- Western blot
Unit Definition
One unit Streptavidin-POD, corresponding to one unit peroxidase, will oxidize 1 μmol of guaiacol tetramer in one minute at +25 °C and pH 7.0.
Unit Conversion: 1 U (+25 °C; guaiacol as substrate; pH7.0) corresponds to 1.24 U (+25 °C; 1.7 mM ABTS as substrate; pH= 6.0) or to 4.0 U (+25 °C; 8.7 mM ABTS as substrate; pH = 5.0).
Unit Conversion: 1 U (+25 °C; guaiacol as substrate; pH7.0) corresponds to 1.24 U (+25 °C; 1.7 mM ABTS as substrate; pH= 6.0) or to 4.0 U (+25 °C; 8.7 mM ABTS as substrate; pH = 5.0).
Physical form
Liquid, stabilized
Preparation Note
Working concentration: 5 - 500 mU/ml (i.e., stock solution can be diluted).Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
- ELISA: 50 to 200 mU/ml
- Immunohistocytochemistry: 250 to 1000 mU/ml
- Western blot: 200 to 500 mU/ml
Other Notes
For life science research only. Not for use in diagnostic procedures.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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