DMEM/nutrient Mixture F-12 Formulation

DMEM/F12 medium is 1:1 mixture of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F-12 Nutrient Mixture. It is serum-free medium that contained insulin, transferrin, epidermal growth factor, leutinizing hormone or follicle stimulating hormone, somatomedin, and growth hormone first reported by Mather and Sato. DMEM/F12 media can also include HEPES buffer at a final concentration of 15 mM to compensate for the loss of buffering capacity from eliminating serum.

Click on the product numbers at the top of each column.

Component	D0547 g/L	D2906 g/L	D6421 [1x] g/L	D6434 [1x] g/L	D8062 [1x] g/L	D8437 [1x] g/L	D8900 g/L	D9785 g/L
Inorganic Salts
Ammonium Molybdate • 4H2O	0.00000618	—	—	—	—	—	—	—
Ammonium Metavandate	0.00000058	—	—	—	—	—	—	—
Calcium Chloride	0.1166	0.1545	0.1166	0.1545	0.1166	0.1545	0.1545	—
Cupric Sulfate • 5H2O	0.0000013	0.0000013	0.0000013	0.0000013	0.0000013	0.0000013	0.0000013	0.0000013
Ferric Nitrate • 9H2O	0.00005	0.00005	0.00005	0.00005	0.00005	0.00005	0.00005	0.00005
Ferrous Sulfate • 7H2O	0.000417	0.000417	0.000417	0.000417	0.000417	0.000417	0.000417	0.000417
Manganese Sulfate	0.000000151	—	—	—	—	—	—	—
Magnesium Chloride • 6H2O	0.0612	0.0612	0.0612	0.0612	0.0612	0.0612	0.0612	—
Magnesium Sulfate (anhydrous)	0.04884	0.04884	0.04884	0.04884	0.04884	0.04884	0.04884	—
Nickel Chloride	0.00000012	—	—	—	—	—	—	—
Potassium Chloride	0.3118	0.3118	0.3118	0.3118	0.3118	0.3118	0.3118	0.3118
Sodium Bicarbonate	—	—	1.2	—	—	1.2	—	—
Sodium Chloride	6.996	6.996	6.996	6.996	6.996	6.996	6.996	6.996
Sodium Metasilicate • 9H2O	0.0000142	—	—	—	—	—	—	—
Sodium Selenite	0.00000519	—	—	—	—	—	—	—
Sodium Phosphate Dibasic (anhydrous)	0.07102	0.07102	0.07102	0.07102	0.07102	0.07102	0.07102	0.07102
Sodium Phosphate Monobasic (anhydrous)	0.0543	0.0543	0.0543	0.0543	0.0543	0.0543	0.0543	0.0543
Stannous Chloride • 2H2O	0.00000011	—	—	—	—	—	—	—
Zinc Sulfate • 7H2O	0.000432	0.000432	0.000432	0.000432	0.000432	0.000432	0.000432	0.000432
Amino Acids
L-Alanine	0.00445	0.00445	0.00445	0.00445	0.00445	0.00445	0.00445	0.00445
L-Arginine • HCl	0.1475	0.1475	0.1475	0.1475	0.1475	0.1475	0.1475	0.1475
L-Asparagine • H2O	0.0075	0.0075	0.0075	0.0075	0.0075	0.0075	0.0075	0.0075
L-Aspartic Acid	0.00665	0.00665	0.00665	0.00665	0.00665	0.00665	0.00665	0.00665
L-Cystine • 2HCl	0.03129	0.03129	0.03129	0.03129	0.03129	0.03129	0.03129	0.03129
L-Cysteine • HCl • H2O	0.01756	0.01756	0.01756	0.01756	0.01756	0.01756	0.01756	0.01756
L-Glutamic Acid	0.00735	0.00735	0.00735	0.00735	0.00735	0.00735	0.00735	0.00735
L-Glutamine	0.365	0.365	—	—	0.365	0.365	0.365	—
Glycine	0.01875	0.01875	0.01875	0.01875	0.01875	0.01875	0.01875	0.01875
L-Histidine • HCl • H2O	0.03148	0.03148	0.03148	0.03148	0.03148	0.03148	0.03148	0.03148
L-Isoleucine	0.05447	0.05447	0.05447	0.05447	0.05447	0.05447	0.05447	0.05447
L-Leucine	0.05905	0.05905	0.05905	0.05905	0.05905	0.05905	0.05905	—
L-Lysine • HCl	0.09125	0.09125	0.09125	0.09125	0.09125	0.09125	0.09125	—
L-Methionine	0.01724	0.01724	0.01724	0.01724	0.01724	0.01724	0.01724	—
L-Phenylalanine	0.03548	0.03548	0.03548	0.03548	0.03548	0.03548	0.03458	0.03548
L-Proline	0.01725	0.01725	0.01725	0.01725	0.01725	0.01725	0.01725	0.01725
L-Serine	0.02625	0.02625	0.02625	0.02625	0.02625	0.02625	0.02625	0.02625
L-Threonine	0.05345	0.05345	0.05345	0.05345	0.05345	0.05345	0.05345	0.05345
L-Tryptophan	0.00902	0.00902	0.00902	0.00902	0.00902	0.00902	0.00902	0.00902
L-Tyrosine • 2Na • 2H2O	0.05579	0.05579	0.05579	0.05579	0.05579	0.05579	0.05579	0.05579
L-Valine	0.05285	0.05285	0.05285	0.05285	0.05285	0.05285	0.05285	0.05285
Vitamins
D-Biotin	0.0000035	0.0000035	0.0000035	0.0000035	0.0000035	0.0000035	0.0000035	0.0000035
Choline Chloride	0.00898	0.00898	0.00898	0.00898	0.00898	0.00898	0.00898	0.00898
Folic Acid	0.00265	0.00265	0.00265	0.00266	0.00266	0.00266	0.00266	0.00266
myo-Inositol	0.0126	0.0126	0.0126	0.0126	0.0126	0.0126	0.0126	0.0126
Niacinamide	0.00202	0.00202	0.00202	0.00202	0.00202	0.00202	0.00202	0.00202
D-Pantothenic Acid (hemicalcium)	0.00224	0.00224	0.00224	0.00224	0.00224	0.00224	0.00224	0.00224
Pyridoxal • HCl	0.002	0.002	—	0.002	0.002	0.002	0.002	0.002
Pyridoxine • HCl	0.002031	0.002031	0.002031	—	—	0.000031	0.000031	0.000031
Riboflavin	0.000219	0.000219	0.000219	0.000219	0.000219	0.000219	0.000219	0.000219
Thiamine • HCl	0.00217	0.00217	0.00217	0.00217	0.00217	0.00217	0.00217	0.00217
Vitamin B12	0.00068	0.00068	0.00068	0.00068	0.00068	0.00068	0.00068	0.00068
Other
D-Glucose	3.15	3.15	3.15	3.15	3.15	3.15	3.15	3.15
HEPES	—	3.5745	3.5745	3.5745	—	3.5745	3.5745	3.5745
Hypoxanthine	0.0021	0.00244	0.0021	0.00244	0.0021	0.00244	0.00244	0.0021
Linoleic Acid	0.000042	0.000042	0.000042	0.000042	0.000042	0.000042	0.000042	0.000042
Phenol Red • Na	0.00863	—	0.00863	—	0.00863	0.00863	0.00863	—
Putrescine • HCl	0.000081	0.000081	0.000081	0.000081	0.000081	0.000081	0.000081	0.000081
Pyruvic Acid • Na	0.11	0.055	0.055	0.055	0.055	0.055	0.055	0.055
DL-Thioctic Acid	0.000105	0.000105	0.000105	0.000105	0.000105	0.000105	0.000105	0.000105
Thymidine	0.000365	0.000365	0.000365	0.000365	0.000365	0.000365	0.000365	0.000365
Add
Sodium Bicarbonate	1.2	1.2	—	—	—	—	1.2	1.2
Calcium Chloride • 2H2O	—	—	—	—	—	—	—	0.1545
L-Glutamine	—	—	0.365	0.365	—	—	—	0.365
L-Leucine	—	—	—	—	—	—	—	0.05905
L-Lysine • HCl	—	—	—	—	—	—	—	0.09125
Magnesium Chloride • 6H2O	—	—	—	—	—	—	—	0.0612
Magnesium Sulfate	—	—	—	—	—	—	—	0.04884
L-Methionine	—	—	—	—	—	—	—	0.0172