HPLC Analysis of Vitamin D2 and D3

Figure 1.Elution Order: Ergocalcifero (Vitamin D2) and Cholecalciferol (Vitamin D3)
Conditions | |
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column | Ascentis® Express C18, 25 cm x 4.6 mm I.D., 5 µm particles (50538-U) |
column temp. | 35 °C |
mobile phase | [A] acetonitrile; [B] methanol; (95:5, A:B) |
flow rate | 1.5 mL/min |
pressure | 1726 psi (119 bar) |
sample | 50 µg/mL in 90:10, acetonitrile:methanol |
injection | 10 µL |
detector | UV, 265 nm |
Description | |
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Analysis Note | This application demonstrates the suitability of the Ascentis Express C18, 5 μm, for the routine analysis of the fat-soluble vitamins D2 and D3 . Structures along with the optimized chromatogram obtained on the Ascentis Express C18, 5 μm are presented. |
Categories | Analytical Chromatography, Vitamins and Natural products, Steroids |
Featured Industry | Clinical Vitamins, Nutraceuticals, and Natural Products |
suitability | application for HPLC |
Materials
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