Feedback inhibition of glycerol kinase, a catabolic enzyme in Escherichia coli.

Feedback inhibition of glycerol kinase, a catabolic enzyme in Escherichia coli.

Science (New York, N.Y.) (1966-08-12)

N Zwaig, E C Lin

ABSTRACT

Fructose-1 ,6-diphosphate is a feedback inhibitor of the catabolic enzyme, glycerol kinase, in Escherichia coli. A mutant was isolated which produced a desensitized enzyme. Glucose was no longer as effective in preventing the utilization of exogenous glycerol by cells which synthesized constitutively such an altered enzyme, even though the usual degree of catabolite repression still operated.

MATERIALI

N° Catalogo

Marchio

Descrizione del prodotto

G6142

Sigma-Aldrich

Glycerokinase from Cellulomonas sp., lyophilized powder, 25-75 units/mg protein

G6278

Sigma-Aldrich

Glycerokinase from Escherichia coli, 300-600 units/mL, ammonium sulfate suspension

G0774

Sigma-Aldrich

Glycerokinase from Bacillus stearothermophilus, buffered aqueous solution, ≥75 units/mg protein (biuret)

G4509

Sigma-Aldrich

Glycerokinase from Escherichia coli, lyophilized powder, 40-100 units/mg protein