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MiR-378b Promotes Differentiation of Keratinocytes through NKX3.1.

MiR-378b Promotes Differentiation of Keratinocytes through NKX3.1.

PloS one (2015-08-28)

Xi-Liang Wang, Tao Zhang, Jing Wang, Dian-Bao Zhang, Feng Zhao, Xue-Wen Lin, Zhe Wang, Ping Shi, Xi-Ning Pang

ABSTRACT

MicroRNA (miRNA) is a kind of short non-coding RNA, involved in various cellular processes. During keratinocyte differentiation, miRNAs act as important regulators. In this study, we demonstrated by microarray assay that the expression of miR-378b significantly increased during keratinocytes differentiation. Our findings showed that miR-378b could inhibit proliferation, migration and differentiation in keratinocytes. Luciferase reporter assays showed that miR-378b directly target NKX3.1. Silencing of NKX3.1 could coincide with the effects of miR-24 overexpression. In conclusion, our results demonstrate miR-378b promote keratinocytes differentiation by targeting NKX3.1. Manipulation of miR-378b may afford a new strategy to clinic treatment of skin injury and repair.

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