Synthesis and LC-MS/MS analysis of desmosine-CH2, a potential internal standard for the degraded elastin biomarker desmosine.

Synthesis and LC-MS/MS analysis of desmosine-CH2, a potential internal standard for the degraded elastin biomarker desmosine.

Organic & biomolecular chemistry (2014-10-31)

Yuko Murakami, Rina Suzuki, Hiroto Yanuma, Jiangtao He, Shuren Ma, Gerard M Turino, Yong Y Lin, Toyonobu Usuki

ABSTRACT

Desmosine-CH2, an analog of the elastic tissue degradation biomarker desmosine, can be regarded as a potential internal standard for precise quantification of desmosines by LC-MS/MS. In this study, the chemical synthesis of desmosine-CH2 was completed in 22% overall yield in five steps. The LC-MS/MS analysis of desmosine-CH2 was also achieved.

MATERIALI

N° Catalogo

Marchio

Descrizione del prodotto

164194

Sigma-Aldrich

Acido eptafluorobutirrico, 98%

77249

Sigma-Aldrich

Acido eptafluorobutirrico, ≥99.0% (GC)

52411

Supelco

Acido eptafluorobutirrico, suitable for ion chromatography, ≥99.5% (GC)

49540

Supelco

Heptafluorobutyric acid solution, 0.5 M in H₂O, LiChropur^™, suitable for ion pair chromatography