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  • Efficiency of NaOCl and laser-assisted photosensitization on the reduction of Enterococcus faecalis in vitro.

Efficiency of NaOCl and laser-assisted photosensitization on the reduction of Enterococcus faecalis in vitro.

Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics (2006-09-26)
Aguinaldo Silva Garcez, Silvia Cristina Núñez, José Luís Lage-Marques, Antonio Olavo Cardoso Jorge, Martha Simões Ribeiro
ABSTRACT

To investigate the action of a red laser associated with a photosensitizer on the reduction of Enterococcus faecalis in dental root canal in vitro. Thirty prepared teeth with single canals were contaminated. The chemical group was irrigated with 0.5% NaOCl and left flooded for 30 minutes. In the laser group, a paste-based photosensitizer was maintained in the root canals for 5 minutes, and then irradiated with a laser at 685 nm using an optical fiber for an E of 1.8 J during 3 minutes. After treatment, the canal content was collected, serially diluted, and cultured to determine the number of colony-forming units. Photosensitizer alone or laser alone did not have any bactericidal effect. Chemical solution reduced viable bacteria in 93.25%. Laser photosensitization resulted in a reduction of 99.2%, a significantly higher bacterial reduction than NaOCl. Laser photosensitization was effective for reducing E. faecalis in root canals and could be an adjunct to endodontic treatment.

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Sigma-Aldrich
Azulene, 99%