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The Notch ligand, Jagged-1, influences the development of primitive hematopoietic precursor cells.

Blood (1998-05-30)
B Varnum-Finney, L E Purton, M Yu, C Brashem-Stein, D Flowers, S Staats, K A Moore, I Le Roux, R Mann, G Gray, S Artavanis-Tsakonas, I D Bernstein
ABSTRACT

We examined the expression of two members of the Notch family, Notch-1 and Notch-2, and one Notch ligand, Jagged-1, in hematopoietic cells. Both Notch-1 and Notch-2 were detected in murine marrow precursors (Lin-Sca-1+c-kit+). The Notch ligand, Jagged-1, was not detected in whole marrow or in precursors. However, Jagged-1 was seen in cultured primary murine fetal liver stroma, cultured primary murine bone marrow stroma, and in stromal cell lines. These results indicate a potential role for Notch-Notch ligand interactions in hematopoiesis. To further test this possibility, the effect of Jagged-1 on murine marrow precursor cells was assessed by coculturing sorted precursor cells (Lin-Sca-1+c-kit+) with a 3T3 cell layer that expressed human Jagged-1 or by incubating sorted precursors with beads coated with the purified extracellular domain of human Jagged-1 (Jagged-1(ext)). We found that Jagged-1, presented both on the cell surface and on beads, promoted a twofold to threefold increase in the formation of primitive precursor cell populations. These results suggest a potential use for Notch ligands in expanding precursor cell populations in vitro.

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Sigma-Aldrich
Jagged-1 (human): FC (human), recombinant, expressed in HEK 293 cells, >90% (SDS-PAGE)