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Cell-Free Reconstitution of Autophagic Membrane Formation.

Methods in molecular biology (Clifton, N.J.) (2019-01-06)
Min Zhang, Liang Ge
ABSTRACT

Autophagy is a catabolic pathway for bulk turnover of cytoplasmic components through the lysosome. Completion of autophagy requires a sophisticated membrane remodeling process. The early steps involve autophagic membrane precursor generation from the intracellular membranes. The intricate protein-membrane interactions underlying autophagic membrane precursor generation have been a focus of attention but yet poorly defined. Here, we summarize the procedure of a cell-free system we have established to dissect the molecular mechanism of early autophagic membrane generation.

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Golgi Isolation Kit, sufficient for 50 g (tissue)
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